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表面活性剂LAS对原生动物压迫效应的研究
蔡俊鹏
Subtype硕士
Thesis Advisor沈韫芬
1986
Degree Grantor中国科学院水生生物研究所
Place of Conferral中国科学院水生生物研究所
Degree Discipline水生生物学
Abstract本文进行了表面活性剂LAS对原生动物的种、种群、群落等三个不同生物学组织水平的毒性试验。结果表明:LAS对5种原生动物的急性毒性作用没有种的特异性(P > 0.05),12h-LC50在7.0-10.47p pm之间,对美州四膜虫种群的急性毒性作用。12h-LC50为17.21p pm;对其呼吸率的影响,应用Gilson差分呼吸仪和极谱氧电极微呼吸仪进行测定,表明其效应浓度分别为λ2.84和9.63p pm (P < 0.05)。3-40p pm LAS对微型生物群落的结构。功能有不同程度的影响。结构参数有原生动物种类组成和种类数;功能参数有原生动物群过程。微型生物群落的P/R比率。反映原生动物群集过程的三个参数Seq、G和T90%中的Seq与LAS浓度的自然对数成直线负相关。由其回归方程y = 29.7281-5.6722x(P < 0.05)推算出LAS对原生动物群落的EC_(50)、EC_(20)和EC_5分别为4.684、 0.51和0.1681p pm。以种、种群水平的12h-LC50为基础,推算出的MATC,在AF为0.1时,分别为0.7和1.721p pm;在AF为0.01时,MATC则为0.07和0.1721p pm。比较来自种、种群水平的MATC与来自群落水平的EC_(20)、EC_5。后者具有更大的科学性和环境真实性。因此,对于东湖,MATC不得高于0.5p pm,最好是低于0.17p pm。
Other AbstractThe toxic tests of surfactant LAS(Linear Alkylbenzene Sulfonate) was carried out at three biological organization levels species populatiocommunity Results showed that there were no specific differences in the acute toxicity of LAS among five protozoan species. Paramecium caudatum, Tetrahymena americanis, T. pigmentosa, T.borealis, 3IV WWO.T.borealis 3II VM665, and T.tropicalis DIV TC89 (P > 0.05). 12h-LC50 was between 7.0 and 10.47 p pm. At the population leve of Tetrahymena americanis, the 12h-LC50 was 17.21 p pm; the effective concentrations of LAS. based on the respiration of Tetrahymena americanis, were 12.84 p pm and 9.63 p pm (P < 0.05). as determine by using the Gilson Differential Respirometer and the polyrographic Respirometer respectively. From low (3p pm) to high (40p pm) concentrations, the toxic effects of LAS on the structure and function of microbial community increased. The structural parameters included the number of protozoan species and their composition. The functional parameters included the protozoan colonization (Seq. G. T90%) and the ratio of photosynthesis to respiration. There was a strong negative correlation between Seq and natural logarithm of LAS concentration with the regression formula of Seq = 39.7281-5.6722 lnC, P < 0.05. The effective concentrations (EC) were calculated by the formula, thus the EC_(50), EC_(20), EC_5 were 4.684, 0.51, 0.1681 p pm respectively. Based on LC50 in species and population levels, MATC were 0.71 and 1.721 p pm when AF was 0.01. Compared the MATC and EC_(20), EC_5, it showed that the results from the microcosms tests have more scientific and environmental realism. Thus, to the Donghu Lake. the MATC must be adjusted below 0.5p pm. below 0.17p pm is even better.
Pages80
Language中文
Document Type学位论文
Identifierhttp://ir.ihb.ac.cn/handle/342005/12700
Collection学位论文
Recommended Citation
GB/T 7714
蔡俊鹏. 表面活性剂LAS对原生动物压迫效应的研究[D]. 中国科学院水生生物研究所. 中国科学院水生生物研究所,1986.
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