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利用多靶位生物标志物评价持久性有机污染物对鱼体的内分泌干扰毒性效应
Alternative TitleThe Use of Multi-Target Biomarkers for Assessment of Endocrine Disruptions of Persistent Organic Pollutants (POPs) in Fish
张捷
Subtype博士
Thesis Advisor徐盈
2002
Degree Grantor中国科学院水生生物研究所
Place of Conferral中国科学院水生生物研究所
Degree Discipline环境科学与工程
Keyword持久性有机污染物 内分泌干扰作用 多靶位生物标志物 毒性作用 水环境鱼体生长发育
Abstract该研究根据持久性有机污染物的毒性作用机制,通过野外及室内暴露实验相结合,利用多靶位生物标志物,建立了污染长期低剂量暴露与鱼体内相应毒性效应间的关系,进而评价了水环境中持久性有机污染物对鱼体生长发育的毒性效应.结果表明,水环境中持久性有机污染物能通过不同作用靶位,作用剂量及暴露周期,干扰鱼体内分泌系统对机体的正常调控作用,从而影响鱼类的生长,代谢,发育,行为及对胁迫的应变能力等多种生理功能.利用多靶位生物标志物,可以全面准确评价水环境中持久性有机污染物长期低剂量暴露对鱼体生长发育的内分泌干扰毒性效应.
Other AbstractThe research described in this thesis concerns the mechanistic aspects of the endocrine disruptive effects of persistent organic pollutants (POPs) on fish growth and development, with the aim to develop multi-target biomarkers for POPs toxic action. The study investigates toxic and biochemical effects of POPs on fish following experimental or environmental exposure. Based on the toxicological mechanisms of actions of POPs, biomarkers are developed and validated to identify causal relationships between exposure to POPs and adverse effects, even at low levels of exposure. In field research, silver carp (Hypophthalmichthys molitrix) samples were collected from five selected sites that represent diverse levels of downgraded persistent organic pollutants contamination in Ya-Er Lake, China. Hepatic Ethoxyresorufin-O-deethylase (EROD) and UDP glucuronosyltransferase (UDPGT) activities, hepatic retinoid, and serum thyroid hormones, were measured. It was found that hepatic retinol and serum free 3, 5, 3'- tetraiodothyronine (FT3) were significantly increasing (p<0.05) when both hepatic EROD and UDPGT activities were significantly declining (p<0.05) from pond 1 to 5 with decrease in the degree of pollution. This significant negative correlation (p<0.01) suggests that the persistent organchlorinated contaminants could induce hepatic EROD and UDPGT activities, alter retinoid and thyroid hormone homeostasis, and finally lead to the reduction of retinol and FT3, the two biologically active forms of retinoid and thyroid hormone in silver carp of Ya-Er Lake. In the laboratory experiments, our studies demonstrate that exposure to 2, 3, 7, 8-TCDD may lead to a decline of thyroid hormones in the early developmental stage of common carp (Cyprinus carpiolinnaeus). It is suggested that disruptions of thyroid function may be used as an effective biomarker to assess the toxic effect of POPs on fish growth and metabolism. It is concluded that persistent organic pollutants, which are involved in the complex regulation of thyroid hormones and retinoid, may have potential physiological, developmental and adverse impacts on fish. In the another short-term exposure experiment, common carps about one-year old were exposed to sediments obtained from contaminated Ya-Er Lake, No. 1 pond, and an unpolluted reference site, Honglian Lake. EROD assays were performed both in vivo and vitro to determine whether EROD assays are sensitive to be used as the early warning system to assess toxic effects of POPs. The results showed both EROD activities in vivo and vitro were increased during the 15 days exposure. The studies demonstrate that combined EROD assays both in vivo and vitro are the effective measurement to monitor toxic process of POPs, such as PCDD/F, PAHs, PCB, HCH, etc,which act through Ah receptor pathway. In vitro investigations, the study was conducted to explore the effects of acute exposure to TCDD on calcium regulation in the cloned human hepatoma cells (Hep-3B). [Ca2+]i levels were detected and quantified by using the calcium indicator fura-2 and digital imaging techniques. The expression of hepatic Ca2+-binding protein regucalcin was investigated after exposure to TCDD in Hep-3B cells. The change in regucalcin mRNA was analyzed by QRT-PCR and IP-RP-HPLC. The present results indicate that TCDD (0.03-3nM) lead to a rapid concentration-dependent increase in [Ca2+]i. The dose-effect relationship between TCDD and [Ca2+]i in Hep 3B> cells according to the following equation: y = -0.1412x2 + 1.2147x - 1.0888, R2 = 0.8672. It also shows that regucalcin mRNA expression in Hep3B cells was reduced by the culture with TCDD (0.01-0.lnM). Further study is needed to investigate on relationship between TCDD dose and regucalcin expression. In summary, the research presented in this thesis shows POPs disrupt the fish endocrine system through different targets, doses and time courses, inducing toxic effects to the fish growth, metabolism, development, and reaction on stress. In view of the recent developments in science, more emphasis should be put on developing appropriate tools to study the role of environments POPs. Multi-target biomarker assay is a useful approach on evaluation endocrine disruption of POPs in aquatic environment.
Pages91
Language中文
Document Type学位论文
Identifierhttp://ir.ihb.ac.cn/handle/342005/12646
Collection学位论文
Recommended Citation
GB/T 7714
张捷. 利用多靶位生物标志物评价持久性有机污染物对鱼体的内分泌干扰毒性效应[D]. 中国科学院水生生物研究所. 中国科学院水生生物研究所,2002.
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