Integrated Vertical-flow Constructed Wetland (IVCW), as a kind of new system, Nitrogen removal mainly depends on these areas by nitrification and denitrification. And denitrification is the key point for nitrogen removal from constructed wetland. Carbon sources become the bottleneck of nitrogen removal in contructed wetland. 1、In IVCW test system with high ammonia nitrogen wastewater treatment process, adding glucose and carboxymethyl cellulose to IVCW system through aeration pipe under 60L•d-1 flow rate, ammonia nitrogen removal rate had no significant difference between adding carbon source group and control group. 2、In IVCW test system with high nitrate wastewater treatment process, nitrate nitrogen removal had significant difference between adding carbon source group and control group. And adding carbon sources can also significantly improve nitrate nitrogen load of IVCW（p =0.019）. Glucose was the better carbon source for IVCW denitrification, and not exceeded the COD in effluent. This study explores the change rule of nitrogen and CODCr under the different quantity of glucose supplement in IVCW effluent. When hydraulic load was 60L•d-1, COD mean concentration was 110mg•L-1, NO3--N mean concentration was 28.8mg•L-1, the dose of 1.5g (25ppm)glucose in IVCW was the optimization for denitrification through aeration pipe of IVCW. C6H12O6 : NO3--N was 4.3 and far lower than that by adding in inflow. 3、Analysis of variance (p<0.01) results indicated that different time of adding carbon source could also cause significant differences of IVCW denitrification. Adding glucose four hours before influent feeding could improve nitrogen removal under this operating condition This paper puts forward a new way of carbon source supplement in IVCW system, using special aeration pipe and blow-down pipe as wetland carbon sources transport pipelines. Adding carbon source (glucose and carboxymethyl cellulose) to the bottom of IVCW could intensify nitrogen removal function of IVCW.