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Alternative TitleStudies on heat shock protein genes in Synechocystis sp.PCC6803
Degree Grantor中国科学院水生生物研究所
Place of Conferral水生生物研究所
Keyword集胞藻pcc 6803 热激蛋白 细菌荧光素酶基因 趋光性 Sll1384
Abstract集胞藻(Synechocystis sp.)PCC6803是一种单细胞蓝藻,既可以依靠光合作用进行自养生长,也可以利用葡萄糖进行异养生长,是蓝藻分子遗传学研究中的重要模式生物。集胞藻PCC6803基因组中有很多热激蛋白基因。本论文对一些热激蛋白基因进行了初步研究。 本论文研究内容和结果分两部分叙述: 一、以细菌荧光素酶基因luxAB和壮观霉素抗性基因逐个插入热激蛋白基因,对其中完全分离的突变株在15℃和40℃下的生长情况进行了测定,发现这些基因突变并不影响集胞藻的生长。对这些突变株在15℃下的细菌荧光素酶活性进行了测定,发现基因sll0430、sll0535和slr0093的转录明显上调。 二、基因sll1384突变株几乎失去趋光性运动,互补实验可恢复其表型。突变株的电镜观察表明,其表面第Ⅳ类纤毛也没有受到影响。推测sll1384编码的蛋白是通过影响信号传递从而在集胞藻的趋光性运动中发挥重要作用。 关键词:集胞藻PCC 6803;热激蛋白;细菌荧光素酶基因;趋光性;sll1384
Other AbstractSynechocystis sp. PCC 6803, a unicellular cyanobacterium, is able to utilize light energy to grow autotrophically or glucose to grow heterotrophically. It is an important model species used in the molecular genetics study of cyanobacteria. Many heat shock protein genes are found in the genome of Synechocystis sp. PCC 6803. In this thesis, some preliminary studies of heat shock protein genes are presented. The researches and results are described in two parts: 1. Heat shock protein genes were each inserted with bacterial luciferase genes luxAB and spectinomycin resistance gene. Of the resulted mutants, some fully segregated were tested for growth at 15oC and 40oC. None of these mutations showed effects on growth of Synechocystis at these temperatures. Assays of luciferase activities in these strains at 15oC indicated that genes sll0430, sll0535 and slr0093 were transcriptionally up-regulated upon transfer of cells to 15oC. 2. The mutant of sll1384 almost completely lost phototaxis motility, and complementation with the wild type gene restored its phenotype. Electron microscopy analysis showed that the type-IV pili remained unchanged on the cell surface. It is hypothesized that sll1384-encoded protein may affect the signal transduction pathway that plays an important role in phototaxis of Synechocystis. Key words: Synechocystis sp. PCC 6803; heat shock proteins; bacterial luciferase genes; phototaxis; sll1384.
Document Type学位论文
Recommended Citation
GB/T 7714
程志. 集胞藻PCC6803热激蛋白基因的研究[D]. 水生生物研究所. 中国科学院水生生物研究所,2009.
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