|Other Abstract||Caenorhabditis elegans, as good model organism, has been used in the study of resistant mechanism against anthelmintics. On the other hand, formalin has been widely used in prevention and cure for fish parasites. However, because of long term application, many parasites generate resistance to formalin. In the present study, after the nematode C. elegans was serially cultivated in the media containing 800ppm formalin for one month, the up-regulation genes were screened by using the methods of suppression subtractive hybridization. Totally 676 gene clones were got. Then, we use dot blot hybridization to screening the positive clones and got 161 gene clones that showed evident expression discrepancy. At last, the clones were sequenced.
BLAST analyses indicate that: (1) Apoptosis related genes’ expression has been up-regulated. These genes encoding proteins include mitochondrial respiratory chain related protein, protein SGT-1 containing TPR motif, heat shock protein, oxidative stress related protein, endocytosis related protein, DNA duplication and reparation related protein and other important apoptosis related protein. (2) Those genes coding important transcription regulation factor and signal transduction related protein, for example, transcriptional activation factor FosB/c-Fos, G protein, cyclin B, Ca-binding protein, and nucleosome assembly protein NAP-1, have been up-regulated expression. (3) Some genes participating in energy metabolism, protein metabolism, fat metabolism and amino acid metabolism have also been up-regulated. (4) In addition, some gene clones coding unnamed protein have been obtained.
The results indicate that besides inducing apoptosis., formalin also changes Caenorhabditis elegans‘ cell metabolism This study establishes foundation for further understanding of the resistance mechanism of organism to formalin.|