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GENE-TRANSFER VIA ELECTROPORATION IN FISH
XIE, YF; LIU, D; ZOU, J; LI, GH; ZHU, ZY; XIE, YF, CHINESE ACAD SCI,INST HYDROBIOL,WUHAN 430072,PEOPLES R CHINA
1993
Conference Name4TH INTERNATIONAL SYMP ON GENETICS IN AQUACULTURE
Source PublicationAQUACULTURE
Pages207-213
Conference DateAPR 29-MAY 03, 1991
Conference PlaceWUHAN, PEOPLES R CHINA
AbstractA fusion human growth hormone gene driven by the mouse metallothionein-1 promoter was successfully introduced into the fertilized eggs of loach and red crucian carp using a capacitor-based electroporator. The results of DNA hybridization showed that the transfer efficiency and the transgene copy number per genome were positively correlated to the voltage, duration and capacity of the pulse. Under optimum conditions, a transfer efficiency of up to 62.5% was achieved. Some fish arising from embryos treated in this manner exhibited over 100 copies of the transgene integrated into their genomes.
KeywordTransgenic Fish Eggs
Funding OrganizationCHINESE ACAD SCI, WUHAN BRANCH, HUBEI PROVINCIAL COMM SCI & TECHNOL, PEOPLES GOVT WUHAN, CHINESE ACAD SCI, INST HYDROBIOL, INT CULTURE EXCHANGE CTR, HUBEI BRANCH, YANTZE RIVER ACAD ECOL, PROFESSOR ZHUFA WU
Indexed BySCI
Language英语
WOS IDWOS:A1993KZ99900022
Citation statistics
Cited Times:17[WOS]   [WOS Record]     [Related Records in WOS]
Document Type会议论文
Identifierhttp://ir.ihb.ac.cn/handle/342005/11875
Collection会议论文
Corresponding AuthorXIE, YF, CHINESE ACAD SCI,INST HYDROBIOL,WUHAN 430072,PEOPLES R CHINA
Recommended Citation
GB/T 7714
XIE, YF,LIU, D,ZOU, J,et al. GENE-TRANSFER VIA ELECTROPORATION IN FISH[C],1993:207-213.
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