| GENE-TRANSFER VIA ELECTROPORATION IN FISH | |
| XIE, YF; LIU, D; ZOU, J; LI, GH; ZHU, ZY; XIE, YF, CHINESE ACAD SCI,INST HYDROBIOL,WUHAN 430072,PEOPLES R CHINA | |
| 1993 | |
| Conference Name | 4TH INTERNATIONAL SYMP ON GENETICS IN AQUACULTURE |
| Source Publication | AQUACULTURE |
| Pages | 207-213 |
| Conference Date | APR 29-MAY 03, 1991 |
| Conference Place | WUHAN, PEOPLES R CHINA |
| Abstract | A fusion human growth hormone gene driven by the mouse metallothionein-1 promoter was successfully introduced into the fertilized eggs of loach and red crucian carp using a capacitor-based electroporator. The results of DNA hybridization showed that the transfer efficiency and the transgene copy number per genome were positively correlated to the voltage, duration and capacity of the pulse. Under optimum conditions, a transfer efficiency of up to 62.5% was achieved. Some fish arising from embryos treated in this manner exhibited over 100 copies of the transgene integrated into their genomes. |
| Keyword | Transgenic Fish Eggs |
| Funding Organization | CHINESE ACAD SCI, WUHAN BRANCH, HUBEI PROVINCIAL COMM SCI & TECHNOL, PEOPLES GOVT WUHAN, CHINESE ACAD SCI, INST HYDROBIOL, INT CULTURE EXCHANGE CTR, HUBEI BRANCH, YANTZE RIVER ACAD ECOL, PROFESSOR ZHUFA WU |
| Indexed By | SCI |
| Language | 英语 |
| WOS ID | WOS:A1993KZ99900022 |
| Citation statistics | |
| Document Type | 会议论文 |
| Identifier | http://ir.ihb.ac.cn/handle/342005/11875 |
| Collection | 会议论文 |
| Corresponding Author | XIE, YF, CHINESE ACAD SCI,INST HYDROBIOL,WUHAN 430072,PEOPLES R CHINA |
| Recommended Citation GB/T 7714 | XIE, YF,LIU, D,ZOU, J,et al. GENE-TRANSFER VIA ELECTROPORATION IN FISH[C],1993:207-213. |
| Files in This Item: | ||||||
| File Name/Size | DocType | Version | Access | License | ||
| GENE-TRANSFER VIA EL（594KB） | 开放获取 | -- | View Application Full Text | |||
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