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题名: Analysis of genetic heterogeneity among five gynogenetic clones of silver crucian carp, Carassius auratus gibelio Bloch, based on detection of RAPD molecular markers
作者: Zhou, L ; Wang, Y ; Gui, JF
会议文集: CYTOGENETICS AND CELL GENETICS
会议名称: 9th International Workshop on the Identification of Transcribed Sequences (TS 99)
会议日期: OCT 28-31, 1999
出版日期: 2000
会议地点: WASHINGTON, D.C.
关键词: POECILIA-FORMOSA ; HYBRID ORIGIN ; ARBITRARY PRIMERS ; UNISEXUAL FISH ; DNA ; PCR ; CONFIRMATION ; CYTOGENETICS ; SALAMANDER ; EVOLUTION
通讯作者: Gui, JF, Chinese Acad Sci, Inst Hydrobiol, State Key Lab Freshwater Ecol & Biotechnol, Wuhan 430072, Peoples R China
收录类别: SCI
部门归属: Chinese Acad Sci, Inst Hydrobiol, State Key Lab Freshwater Ecol & Biotechnol, Wuhan 430072, Peoples R China
主办者: US DOE
摘要: The gynogenetic silver crucian carp, Carassius auratus gibelio, is a unique model system for studying evolutionary genetics and selective breeding, owing to its specific genetic background and reproductive modes. Five gynogenetic clones were analyzed by the random amplified polymorphic DNA (RAPD) technique, using 30 10-nucleotide-long primers. Twenty-six primers produced well-amplified DNA fragments with reproducible banding patterns, and 24 primers were polymorphic. Nearly identical banding patterns were observed among individuals within each clone, suggesting that each clone might possess a specific pattern owing to its gynogenesis. In contrast, the RAPD patterns of the five clones differed from each other. A phylogenetic tree was constructed using UPGMA cluster analysis based on a total of 3,744 distinguishable fragments (156 per individual). Average genetic distances within and among the five clones clearly indicated their intraclonal homogeneity, interclonal heterogeneity, and phylogenetic relationships. Clones A and P were the most closely related, whereas the most divergence was seen between clone D and clone E or F. A total of 88 polymorphic fragments were scored from 24 primers after excluding bands that were monomorphic for the five clones. Most primers corresponding to the polymorphic fragments amplified reproducible markers specific for one clone or that were shared by two, three, or four clones. Several primers (e.g., Opj-1, Opj-7, and Opp-10) produced abundant banding patterns that could be used to discriminate between the five clones. Markers specific for one or two clones were also identified. The RAPD markers identified in this study will likely benefit evolutionary genetics and selective breeding studies. Copyright (C) 2000 S. Karger AG, Basel.
语种: 英语
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内容类型: 会议论文
URI标识: http://ir.ihb.ac.cn/handle/342005/11849
Appears in Collections:中科院水生所知识产出(2009年前)_会议论文

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Recommended Citation:
Zhou, L; Wang, Y; Gui, JF.Analysis of genetic heterogeneity among five gynogenetic clones of silver crucian carp, Carassius auratus gibelio Bloch, based on detection of RAPD molecular markers.见:.CYTOGENETICS AND CELL GENETICS,,2000,133-139
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