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Use of protein phosphatase inhibition assay to detect microcystins in Donghu Lake and a fish pond in China
Xu, LH; Lam, PKS; Chen, JP; Xu, JM; Wong, BSF; Zhang, YY; Wu, RSS; Harada, KI; Chen, JP, City Univ Hong Kong, Ctr Environm Sci & Technol, Dept Biol & Chem, Tat Chee Ave, Kowloon, Hong Kong, Peoples R China
2000
Conference NameConference on Environmental Contamination, Toxicology and Health
Source PublicationCHEMOSPHERE
Pages53-58
Conference DateSEP 23-25, 1998
Conference PlaceHONG KONG, PEOPLES R CHINA
DepartmentCity Univ Hong Kong, Ctr Environm Sci & Technol, Dept Biol & Chem, Kowloon, Hong Kong, Peoples R China; Chinese Acad Sci, Inst Hydrobiol, Wuhan, Peoples R China; Meijo Univ, Fac Pharm, Nagoya, Aichi 468, Japan
AbstractSeasonal variations in the level of total microcystins in water samples collected from Donghu Lake and a fish pond in Wuhan, China, were studied between March 1995 and February 1996 using a protein phosphatase inhibition assay involving a radioactive P-32-labelled substrate. The assay is highly reliable and repeatable, and is probably the most sensitive assay for microcystin detection to date. Results of the survey indicated the presence of microcystins in the water samples, and the concentration of microcystins appeared to be related to the degree of eutrophication and water temperature. There is also a correlative relationship between the quantity of microcystins and the abundance of microcystin-producing cyanobacteria (Anabaena and Oscillatoria) in the water bodies over a year cycle. In the present study, the positive detection of microcystins in water bodies having no signs of algal bloom warns of considerable potential threat of these waters to public health. (C) 2000 Elsevier Science Ltd. All rights reserved.
KeywordCyanobacteria Toxins Water Lr
Funding OrganizationCroucher Fdn, Environm & Conservat Fund, Hong Kong Baptist Univ, British Council, Dept Trade & Ind, Zhongshan Univ, Perkin Elmer Hong Kong Ltd, Guyline (Asia) Ltd
Indexed BySCI
Language英语
WOS IDWOS:000086786500008
Citation statistics
Cited Times:26[WOS]   [WOS Record]     [Related Records in WOS]
Document Type会议论文
Identifierhttp://ir.ihb.ac.cn/handle/342005/11847
Collection会议论文
Corresponding AuthorChen, JP, City Univ Hong Kong, Ctr Environm Sci & Technol, Dept Biol & Chem, Tat Chee Ave, Kowloon, Hong Kong, Peoples R China
Recommended Citation
GB/T 7714
Xu, LH,Lam, PKS,Chen, JP,et al. Use of protein phosphatase inhibition assay to detect microcystins in Donghu Lake and a fish pond in China[C],2000:53-58.
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