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Induced meiotic gynogenesis in tench, Tinca tinca (L.) using irradiated heterogenic sperm
Wang, JX; Liu, HJ; Min, WQ; Tong, JG; Guan, M; Han, YZ; Gong, LJ; Huang, Z; Ren, J; Zhang, JP; Zheng, HP; Wang, JX, Hubei Prov Fisheries Res Inst, 116 Donghu Rd, Wuchang 430071, Hubei, Peoples R China
2006
Conference Name4th International Workshop on Biology and Culture of the Tench Tinca
Source PublicationAQUACULTURE INTERNATIONAL
Pages35-42
Conference DateSEP 20-23, 2004
Conference PlaceWierzba, POLAND
DepartmentHubei Prov Fisheries Res Inst, Wuchang 430071, Hubei, Peoples R China; Acad Sinica, Inst Hydrobiol, Wuhan 430072, Peoples R China; Zhaoqing Goldeatech Co Ltd, Zhaoqing 526200, Peoples R China
AbstractEffects of the starting time and duration of cold shock, as well as the source of heterogenic sperm on the percentage of viable gynogenic larvae (PVGL) in tench were studied. The DNA in sperm of red common carp (Cyprinus carpio var singuonensis) was inactivated by ultraviolet radiation prior to use to induce meiotic gynogenetic development in tench. In experiment 1, tench eggs were cold-shocked for 30 min starting at 1, 3, 5, 7, 10 and 15 min post activation. In experiment 2, cold shock began 5 min after activation and lasted for 10, 20, 30, 40, 50 min, respectively. Each experiment was run in triplicate using 3 tench females, and one group not treated with cold shock was included in each experiment to serve as a control group. In experiment 3, sperm of bigmouth buffalo (Ictiobus cyprinellus), red common carp or grass carp (Ctenopharyngodon idella) was used to induce gynogenesis in tench with a 20-min cold shock starting 5 min post activation. The results showed that PVGL from the control group was very low (0.11-0.47%). In experiment 1, the highest average PVGL (9.60%) was observed when cold shock treatment was applied 5 min post activation. When cold shock treatment was started 5 min post activation, duration of cold shock affected PVGL. Cold shock lasting 20 min resulted in the highest average PVGL (12. 57%) among the selected duration of cold shock studied in experiment 2. The average PVGL was 2.3, 8.6, and 9.3%, respectively, for eggs induced by sperm of bigmouth buffalo, red common carp and grass carp. Average PVGL was significantly lower for eggs induced by sperm of bigmouth buffalo, compared with that for eggs induced by sperm of the other two species. However, average PVGL were similar for eggs induced by sperm of red common carp and grass carp. In summary, the optimal conditions for gynogenesis in tench include the use of irradiated sperm of grass carp to activate the eggs and cold shock of 20 min starting 5 min post activation. Since female tench grow much faster to a larger size than male tench, gynogenesis of tench holds great potential for production enhancement.
KeywordHeterogenic Sperm Meiotic Gynogenesis Tinca Tinca
Funding OrganizationStanisLaw Sakowicz Inland Fisheries Inst
Indexed BySCI
Language英语
WOS IDWOS:000234692500005
Citation statistics
Cited Times:10[WOS]   [WOS Record]     [Related Records in WOS]
Document Type会议论文
Identifierhttp://ir.ihb.ac.cn/handle/342005/11781
Collection会议论文
Corresponding AuthorWang, JX, Hubei Prov Fisheries Res Inst, 116 Donghu Rd, Wuchang 430071, Hubei, Peoples R China
Recommended Citation
GB/T 7714
Wang, JX,Liu, HJ,Min, WQ,et al. Induced meiotic gynogenesis in tench, Tinca tinca (L.) using irradiated heterogenic sperm[C],2006:35-42.
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