| 题名: | Induced meiotic gynogenesis in tench, Tinca tinca (L.) using irradiated heterogenic sperm |
| 作者: | Wang, JX
; Liu, HJ
; Min, WQ
; Tong, JG
; Guan, M
; Han, YZ
; Gong, LJ
; Huang, Z
; Ren, J
; Zhang, JP
; Zheng, HP
|
| 会议文集: | AQUACULTURE INTERNATIONAL
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| 会议名称: | 4th International Workshop on Biology and Culture of the Tench Tinca
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| 会议日期: | SEP 20-23, 2004
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| 出版日期: | 2006
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| 会议地点: | Wierzba, POLAND
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| 关键词: | heterogenic sperm
; meiotic gynogenesis
; Tinca tinca
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| 通讯作者: | Wang, JX, Hubei Prov Fisheries Res Inst, 116 Donghu Rd, Wuchang 430071, Hubei, Peoples R China
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| 收录类别: | SCI
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| 部门归属: | Hubei Prov Fisheries Res Inst, Wuchang 430071, Hubei, Peoples R China; Acad Sinica, Inst Hydrobiol, Wuhan 430072, Peoples R China; Zhaoqing Goldeatech Co Ltd, Zhaoqing 526200, Peoples R China
|
| 主办者: | StanisLaw Sakowicz Inland Fisheries Inst
|
| 摘要: | Effects of the starting time and duration of cold shock, as well as the source of heterogenic sperm on the percentage of viable gynogenic larvae (PVGL) in tench were studied. The DNA in sperm of red common carp (Cyprinus carpio var singuonensis) was inactivated by ultraviolet radiation prior to use to induce meiotic gynogenetic development in tench. In experiment 1, tench eggs were cold-shocked for 30 min starting at 1, 3, 5, 7, 10 and 15 min post activation. In experiment 2, cold shock began 5 min after activation and lasted for 10, 20, 30, 40, 50 min, respectively. Each experiment was run in triplicate using 3 tench females, and one group not treated with cold shock was included in each experiment to serve as a control group. In experiment 3, sperm of bigmouth buffalo (Ictiobus cyprinellus), red common carp or grass carp (Ctenopharyngodon idella) was used to induce gynogenesis in tench with a 20-min cold shock starting 5 min post activation. The results showed that PVGL from the control group was very low (0.11-0.47%). In experiment 1, the highest average PVGL (9.60%) was observed when cold shock treatment was applied 5 min post activation. When cold shock treatment was started 5 min post activation, duration of cold shock affected PVGL. Cold shock lasting 20 min resulted in the highest average PVGL (12. 57%) among the selected duration of cold shock studied in experiment 2. The average PVGL was 2.3, 8.6, and 9.3%, respectively, for eggs induced by sperm of bigmouth buffalo, red common carp and grass carp. Average PVGL was significantly lower for eggs induced by sperm of bigmouth buffalo, compared with that for eggs induced by sperm of the other two species. However, average PVGL were similar for eggs induced by sperm of red common carp and grass carp. In summary, the optimal conditions for gynogenesis in tench include the use of irradiated sperm of grass carp to activate the eggs and cold shock of 20 min starting 5 min post activation. Since female tench grow much faster to a larger size than male tench, gynogenesis of tench holds great potential for production enhancement. |
| 语种: | 英语
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| Citation statistics: |
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| 内容类型: | 会议论文
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| URI标识: | http://ir.ihb.ac.cn/handle/342005/11781
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| Appears in Collections: | 中科院水生所知识产出（2009年前）_会议论文
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| Induced meiotic gynogenesis in tench.pdf（268KB） | -- | -- | 开放获取 | -- | View
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| Recommended Citation: | |
Wang, JX; Liu, HJ; Min, WQ; Tong, JG; Guan, M; Han, YZ; Gong, LJ; Huang, Z; Ren, J; Zhang, JP; Zheng, HP.Induced meiotic gynogenesis in tench, Tinca tinca (L.) using irradiated heterogenic sperm.见：.AQUACULTURE INTERNATIONAL,,2006,35-42
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