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单链抗体A1-甲基对硫磷水解酶融合蛋白及其制备方法
戴和平; 张先恩; 周亚凤; 杨 薇; 张晓华; 戴玲芬; 张治平
2008-12-31
Rights Holder中国科学院水生生物研究所
Date Available2010-11-03
Country中国
Subtype发明
Abstract本发明公开了一种识别对虾白斑杆状病毒单链抗体Al-甲基对硫磷水解酶融合蛋白及其制备方法,涉及水产养殖生物病毒抗体。单链抗体基因与甲基对硫磷水解酶基因通过与一编码[-(Gly-Ser)↓[5]-]小肽的寡核苷酸片段互补链连接,形成MPH-L-A1E的融合基因,然后再克隆到一大肠杆菌的表达载体上,融合蛋白以可分泌的形式表达。用该融合蛋白可直接与病毒结合,并产生颜色反应,由此可以省去用识别对虾白斑综合症病毒的单链抗体Al检测病毒时必须的二抗操作步骤,简化了操作程序,并降低了检测成本。
Application Date2005-12-16
Language中文
Status授权
Application NumberCN200510020017.4
Document Type专利
Identifierhttp://ir.ihb.ac.cn/handle/342005/11500
Collection专利
Recommended Citation
GB/T 7714
戴和平,张先恩,周亚凤,等. 单链抗体A1-甲基对硫磷水解酶融合蛋白及其制备方法. ZL200510020017.4[P]. 2008-12-31.
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