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专利名称: method for preparing a sterile transgenic carp
发明人: Hu W(胡炜) ; Zhu ZY(朱作言) ; Wang YP(汪亚平) ; Li SF(黎双飞)
专利授权号: US7230156B2
申请日期: 2005-03-31
授权日期: 2007-06-12
专利权人: 中国科学院水生生物研究所
专利类别: 发明
授予国别: 美国
公开日期: 2010-11-03
状态: 授权
摘要: The present invention discloses a preparation method of sterile transgenic fish, and said method includes the following steps: using carp actin gene promoter as promotor, using antisense 296 bp carp salmon type gonadotropin-releasing hormone gene complementary DNA fragment [including sGnRH+peptide and gonadotropin-releasing hormone related peptide coding zone and 3' non-coding zone sequence of 64 bp] as target gene, and using grass carp growth hormone gene 3' flanking sequence as termination sequence to construct salmon type gonadotropin-releasing hormone antisense RNA expression vector, and adopting microinjection method to introduce the recombinant DNA fragment into carp fertilized egg, and utilizing PCR reaction and radioimmunodetection to screen and obtain the invented sterile transgenic fish.
语种: 英语
内容类型: 专利
URI标识: http://ir.ihb.ac.cn/handle/342005/11494
Appears in Collections:中科院水生所知识产出(2009年前)_专利

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胡炜;朱作言;汪亚平;黎双飞,method for preparing a sterile transgenic carp,,2005-03-31
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