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鳜鱼弹状病毒毒株及制备方法和应用
张奇亚; 陶建军; 李正秋; 桂建芳
2007-06-20
Rights Holder中国科学院水生生物研究所
Date Available2010-11-03
Country中国
Subtype发明
Abstract本发明公开了一种鳜鱼弹状病毒毒株及制备方法和应用,鳜鱼弹状病毒毒株Siniperca chuatsi rhabdovirus,SCRV0208,CCTCC NO:V202008,首先是GCF0208细胞经TC199培养基加牛血清,培养一定时间,长成单层细胞,用病毒液接种后,不同时段取样,切片,通过电镜观察确定挑斑时间,挑斑后再接种到单层细胞中,扩大培养,离心后获得提纯的病毒。本发明方法易行,有效地将弹状病毒与之伴随感染的病毒分开,获得高纯度的病毒抗原,在细胞工程疫苗和基因工程疫苗中的应用。
Application Date2003-11-13
Language中文
Status授权
Application NumberCN200310116241.4
Document Type专利
Identifierhttp://ir.ihb.ac.cn/handle/342005/11488
Collection专利
Recommended Citation
GB/T 7714
张奇亚,陶建军,李正秋,等. 鳜鱼弹状病毒毒株及制备方法和应用. ZL200310116241.4[P]. 2007-06-20.
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