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大菱鲆弹状病毒毒株及其制备方法和应用
张奇亚; 李正秋; 桂建芳
2006-02-01
Rights Holder中国科学院水生生物研究所
Date Available2010-11-03
Country中国
Subtype发明
Abstract本发明公开了一种大菱鲆弹状病毒毒株及其制备方法和应用,大菱鲆弹状病毒毒株Scophthalmus maximus rahbdovirus,SMRV0207,CCTCC NO:V202006。将患病大菱鲆的肝、肾、心、脾组织取出后,剪碎、加入PBS进行匀浆,再加入双抗,置冰箱冰冻,取出融化后,离心,再将病毒粗提液经过滤后,分别接种到CLC0207细胞中,恒温继续培养。本方法易行,该病毒株在CLC0207细胞中能迅速增殖,增殖效价可达到108TCID50/ml,该病毒株可应用于海水鱼弹状病毒细胞工程疫苗制备。
Application Date2003-11-13
Language中文
Status授权
Application NumberCN200310116250.3
Document Type专利
Identifierhttp://ir.ihb.ac.cn/handle/342005/11468
Collection专利
Recommended Citation
GB/T 7714
张奇亚,李正秋,桂建芳. 大菱鲆弹状病毒毒株及其制备方法和应用. ZL200310116250.3[P]. 2006-02-01.
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