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牙鲆淋巴囊肿病毒毒株及其制备方法和应用
张奇亚; 李正秋; 袁秀平; 桂建芳
2006-02-01
Rights Holder中国科学院水生生物研究所
Date Available2010-11-03
Country中国
Subtype发明
Abstract本发明公开一种牙鲆淋巴囊肿病毒毒株及其制备方法和应用,牙鲆淋巴囊肿病毒毒株LCDV011126,CCTCC NO:V202007,其步骤是将以患有淋巴囊肿病的牙鲆肿瘤组织取出后,剪碎、加入磷酸缓冲液进行匀浆,加入双抗后,置冰箱冰冻,取出融化后,离心获病毒粗提液,过滤后,分别接种到淡水鱼类细胞中,恒温继续培养,观察细胞病毒,收集细胞,固定、超薄切片和染色后进行观察。本发明方法过程简单,易于调节和控制,成本低,可大量扩增淋巴囊肿病毒,提供所需的病毒材料。
Application Date2003-11-13
Language中文
Status授权
Application NumberCN200310116249.0
Document Type专利
Identifierhttp://ir.ihb.ac.cn/handle/342005/11466
Collection专利
Recommended Citation
GB/T 7714
张奇亚,李正秋,袁秀平,等. 牙鲆淋巴囊肿病毒毒株及其制备方法和应用. ZL200310116249.0[P]. 2006-02-01.
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