IHB OpenIR  > 专利
一种水华蓝藻制备藻蓝蛋白的方法
刘永定; 沈银武; 金传荫; 沈强
2006-06-14
Rights Holder中国科学院水生生物研究所
Date Available2010-11-03
Country中国
Subtype发明
Abstract本发明公开了一种水华蓝藻制备藻蓝蛋白的方法,以鲜藻为原料,首先提取粗滤液,鲜藻脱水后,加入缓冲液,混匀,然后冰冻,冻结后,取出置室温下融溶,冻融3-5次,用板框过滤;其次是超滤纯化,用截留分子量为1000-20万道尔顿的中空纤维超滤器,经过微滤的粗提液使分子量较小的各种物质,透过超滤膜而被清除;第三是静置-澄清纯化,经过超滤纯化的藻蓝蛋白的部分,除去大部分小分子,同时进行浓缩,经浓缩的藻蓝蛋白部分在一定温度保藏;第四是羟基磷灰石柱层析纯化。本发明方法简便,纯化和浓缩效率高,具有社会、环境和经济效益。
Application Date2004-03-27
Language中文
Status授权
Application NumberCN200410012934.3
Document Type专利
Identifierhttp://ir.ihb.ac.cn/handle/342005/11456
Collection专利
Recommended Citation
GB/T 7714
刘永定,沈银武,金传荫,等. 一种水华蓝藻制备藻蓝蛋白的方法. ZL200410012934.3[P]. 2006-06-14.
Files in This Item: Download All
File Name/Size DocType Version Access License
一种水华蓝藻制备藻蓝蛋白的方法.pdf(404KB) 开放获取--View Download
Related Services
Recommend this item
Bookmark
Usage statistics
Export to Endnote
Google Scholar
Similar articles in Google Scholar
[刘永定]'s Articles
[沈银武]'s Articles
[金传荫]'s Articles
Baidu academic
Similar articles in Baidu academic
[刘永定]'s Articles
[沈银武]'s Articles
[金传荫]'s Articles
Bing Scholar
Similar articles in Bing Scholar
[刘永定]'s Articles
[沈银武]'s Articles
[金传荫]'s Articles
Terms of Use
No data!
Social Bookmark/Share
File name: 一种水华蓝藻制备藻蓝蛋白的方法.pdf
Format: Adobe PDF
This file does not support browsing at this time
All comments (0)
No comment.
 

Items in the repository are protected by copyright, with all rights reserved, unless otherwise indicated.