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一种培养发菜细胞的方法
毕永红; 胡征宇; 徐敏; 韩丹翔; 邓中洋; 罗玮; 赵先富; 陈珊; 梅洪
2004-11-03
Rights Holder中国科学院水生生物研究所
Date Available2010-11-03
Country中国
Subtype发明
Abstract本发明公开了一种培养发菜细胞的方法,1.培养液的配制:将配制好的贮藏液加入水中的顺序是,硝酸钠、磷酸氢二钾、七水硫酸镁、二水氯化钙、九水硅酸钠、微量金属元素溶液PIV、土壤浸出液按比例配制;2.藻细胞匀浆的制备,首先将野生发菜丝状体用乙醇和蒸馏水洗涤,无菌培养液浸泡8-10h;其次是用玻璃匀浆器将丝状体匀浆破碎,匀浆液用于细胞培养;3.藻种无菌纯化;4.藻细胞培养;5.实时监控;6.收获;7.干燥;8.继续培养。本发明方法简单,操作方便,安全无污染,生长周期短,生产效率高,营养成分全面,营养含量高,广泛适用于城乡规模化生产培养。
Subject Area生物学
Application Date2002-07-26
Language中文
Status授权
Application NumberCN02138828.8
Document Type专利
Identifierhttp://ir.ihb.ac.cn/handle/342005/11432
Collection专利
Recommended Citation
GB/T 7714
毕永红,胡征宇,徐敏,等. 一种培养发菜细胞的方法. ZL02138828.8[P]. 2004-11-03.
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