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类雌激素毒性快速检测试剂盒的制备方法
徐盈; 梁勇
2004-10-06
Rights Holder中国科学院水生生物研究所
Date Available2010-11-03
Country中国
Subtype发明
Abstract本发明公开了类雌激素毒性快速检测试剂盒的制备方法,首先是卵黄蛋白原的诱导和分离纯化,鱼体腹腔注射乙炔基雌二醇,用离子交换柱分离纯化鱼血清,透析脱盐,制备其冻干粉;其次是卵黄蛋白原多克隆抗体的制备,溶解冻干粉,加入弗氏佐剂乳化,免疫大白兔,制备多克隆抗体;第三是生物素标记的卵黄蛋白原多克隆抗体的制备,亲和柱分离纯化抗体,加入生物素酰-N-羟基丁二酰亚胺酯反应,在PBS缓冲液中透析,加入甘油,冻存。本发明可灵敏、准确、方便的定量检测多种鲤科鱼类血液中、肝组织、肝细胞培养基及鱼体中的卵黄蛋白原。
Subject Area生物学
Application Date2002-08-12
Language中文
Status授权
Application NumberCN02138914.4
Document Type专利
Identifierhttp://ir.ihb.ac.cn/handle/342005/11426
Collection专利
Recommended Citation
GB/T 7714
徐盈,梁勇. 类雌激素毒性快速检测试剂盒的制备方法. ZL02138914.4[P]. 2004-10-06.
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