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一种纯化鲀毒素的方法
金传荫; 刘永定; 宋立荣; 朱家明
2004-08-18
Rights Holder中国科学院水生生物研究所
Date Available2010-11-03
Country中国
Subtype发明
Abstract本发明公开了一种纯化鲀毒素的方法。首先是离子交换层析,使用弱酸性阳离子交换树脂柱,转换成铵型或氢型,将经过澄清的鲀毒素粗提液上柱,以0.1-2N浓度的乙酸一步或分步洗脱,或使用乙酸梯度洗脱,收集含鲀毒素的洗脱液,减压蒸发至干,然后重溶于乙酸中;其次是凝胶过滤层析,将第一步所得液上柱,用乙酸洗脱,收集含鲀毒素的洗脱液,减压蒸发至干,得纯鲀毒素的乙酸盐固体。将上述固体溶于所需盐的相应酸中,蒸干后得所需盐。溶于苦味酸中,制成苦味酸盐,水溶后用NH4OH沉淀,即获得极纯粹的毒素。该方法简单,操作方便,所得纯鲀毒素的纯度符合药用剂型的需要,又能生产色谱试剂。
Subject Area生物学
Application Date2001-05-15
Language中文
Status授权
Application NumberCN01114201.4
Document Type专利
Identifierhttp://ir.ihb.ac.cn/handle/342005/11422
Collection专利
Recommended Citation
GB/T 7714
金传荫,刘永定,宋立荣,等. 一种纯化鲀毒素的方法. ZL01114201.4[P]. 2004-08-18.
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