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一种鱼类电脉冲精子转基因方法
朱作言; 钟家玉
2004-07-28
Rights Holder中国科学院水生生物研究所
Date Available2010-11-03
Country中国
Subtype发明
Abstract本发明公开了一种优化的鱼类电脉冲精子转基因方法,该方法基于电脉冲处理将使外源基因和精子外膜结合更紧密的原理,将电脉冲转基因法和精子介导转基因法相结合,对混有外源基因,并保存在保存液中的精子进行电脉冲处理,再与卵子受精,可使转基因的成功率达到19.6%~46.8%。对5月龄的草鱼尾鳍通过此法,经PCR检测得知,有35.7%的个体中含有外源基因。本方法具有简便、易行、高效、便宜和适用范围广等优点,对于卵膜不易去除,或卵很脆弱的鱼类,尤其适用。
Subject Area生物学
Application Date2001-05-16
Language中文
Status授权
Application NumberCN01114202.2
Document Type专利
Identifierhttp://ir.ihb.ac.cn/handle/342005/11420
Collection专利
Recommended Citation
GB/T 7714
朱作言,钟家玉. 一种鱼类电脉冲精子转基因方法. ZL01114202.2[P]. 2004-07-28.
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