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类雌激素毒性快速检测试片的制备方法
徐盈; 杨方星; 梁勇
2005-02-02
Rights Holder中国科学院水生生物研究所
Date Available2010-11-03
Country中国
Subtype发明
Abstract本发明公开了一种类雌激素毒性快速检测试片的制备方法,该试片能够实现对类雌激素毒性的快速在线检测,它包括下列步骤:首先是对抗原的诱导和分离纯化,离心获得血清,将VTG蛋白溶液脱盐冻干成为冻干粉;其次是抗体的诱导,获得抗血清;再是抗体的分离纯化,分离后的卵黄蛋白原抗体除盐后冻干;第四是胶体金—抗体结合物的制备;第五是胶体金—抗体结合物的吸附;第六是抗原和抗体的固化;第七是试片的装配。本发明方法简单,操作方便,试片体积小,便于携带和使用,适合环保、科研、商检及食品水产等单位进行类雌激素污染检测。
Subject Area生态毒理学
Application Date2002-04-18
Language中文
Status授权
Application NumberCN02115727.8
Document Type专利
Identifierhttp://ir.ihb.ac.cn/handle/342005/11386
Collection专利
Recommended Citation
GB/T 7714
徐盈,杨方星,梁勇. 类雌激素毒性快速检测试片的制备方法. ZL02115727.8[P]. 2005-02-02.
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