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一种藻蓝蛋白的制备方法
金传荫; 刘永定; 王高鸿; 沈强; 何家菀; 宋立荣
2000-10-04
Rights Holder中国科学院水生生物研究所
Date Available2010-11-03
Country中国
Subtype发明
Abstract本发明公开了一种藻蓝蛋白的制备方法,以鲜藻为原料,首先提取粗滤液,鲜藻脱水后,加入缓冲液,混匀,然后置-20℃~-10℃冰冻,冻结后,取出置室温下融溶,冻融3—5次,用板框过滤;其次是超滤纯化,用截留分子量为十万道尔顿的超滤膜,经过微滤的粗提液使分子量在十万道尔顿以下各种物质,透过超滤膜而被清除;第三是静置-澄清纯化,经过超滤纯化的藻蓝蛋白的分部,除去分子量十万道尔顿以下小分子,同时进行浓缩,经浓缩的藻蓝蛋白分部在3—5℃保藏;第四是羟基磷灰石柱层析纯化。本发明方法简便,纯化和浓缩效率高,具有社会、环境和经济效益。
Application Date1999-03-26
Language中文
Status授权
Application NumberCN99116402.4
Document Type专利
Identifierhttp://ir.ihb.ac.cn/handle/342005/11350
Collection专利
Recommended Citation
GB/T 7714
金传荫,刘永定,王高鸿,等. 一种藻蓝蛋白的制备方法. ZL99116402.4[P]. 2000-10-04.
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