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Purification and characterization of White Spot Syndrome Virus (WSSV) produced in an alternate host: crayfish, Cambarus clarkii
Can-hua Huang; Li-ren Zhang; Jian-hong Zhang; Lian-chun Xiao; Qing-jiang Wu; Di-hua Chen; Joseph K.-K. Li; Chen, DH, Acad Sinica, Wuhan Inst Virol, Joint Lab Invertebrate Virol, Wuhan 430071, Peoples R China
2001
Source PublicationVIRUS RESEARCH
Volume76Issue:2Pages:115-125
AbstractPenaeid shrimp is the natural host of White Spot Syndrome Virus (WSSV) that can cause high mortality in the infected hosts. Attempts to obtain sufficient amounts of purified intact WSSV for characterization have been unsuccessful. Using crayfish, Cambarus clarkii as a proliferation system, a large amount of infectious WSSV was reproduced and intact WSSV viral particles were purified with a new isolation medium by ultra-centrifugation. Purified WSSV particles were very sensitive to organic solvents and the detergent, Triton X-100. The size of the rod-shape, somewhat elliptical, intact WSSV was 110-130 x 260-350 mm with a long, tail-like envelope extension. The naked viral nucleocapsid was about 80 x 350 nm, and it possessed 15 spiral and cylindrical helices composed of 14 globular capsomers along its long axis, and a 'ring' structure at one terminus. Distinct WSSV genome DNA patterns were obtained when the purified genomic dsDNA of WSSV was digested with five different restriction enzymes (HindIII, XhoI, B(BamHI, SalI, and SacI). In addition, at least 13 major and distinct protein bands could be observed when purified intact WSSV viruses were separated by SDS-PAGE followed by Coomassie Brilliant R-250 staining. The estimated molecular weights of these proteins were 190, 84, 75, 69, 68, 58, 52, 44, 28, 27.5, 23, 19, and 16 kD, respectively. Both the 44 and 190 kD proteins were easily removed if the hemolymph from the: WSSV infected crayfish was transiently treated with 1%, Triton X-100 before it was subjected to gradient centrifugation, indicating that both of them are located on the surface of the viral envelope. These characteristics are consistent with WSSV isolated from the penaeid shrimp. (C) 2001 Elsevier Science B.V. All rights reserved.
KeywordWhite Spot Syndrome Virus Crayfish Host Electron Microscopy Wssv Viral Genome And Proteins
DepartmentAcad Sinica, Wuhan Inst Virol, Joint Lab Invertebrate Virol, Wuhan 430071, Peoples R China; Acad Sinica, Inst Hydrobiol, State Key Lab Freshwater Ecol & Biotechnol, Wuhan 430072, Peoples R China; Utah State Univ, Dept Biol, Logan, UT 84322 USA
Subject AreaVirology
Indexed BySCI
WOS IDWOS:000169675300001
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Document Type期刊论文
Identifierhttp://ir.ihb.ac.cn/handle/152342/9976
Collection期刊论文
Corresponding AuthorChen, DH, Acad Sinica, Wuhan Inst Virol, Joint Lab Invertebrate Virol, Wuhan 430071, Peoples R China
Recommended Citation
GB/T 7714
Can-hua Huang,Li-ren Zhang,Jian-hong Zhang,et al. Purification and characterization of White Spot Syndrome Virus (WSSV) produced in an alternate host: crayfish, Cambarus clarkii[J]. VIRUS RESEARCH,2001,76(2):115-125.
APA Can-hua Huang.,Li-ren Zhang.,Jian-hong Zhang.,Lian-chun Xiao.,Qing-jiang Wu.,...&Chen, DH, Acad Sinica, Wuhan Inst Virol, Joint Lab Invertebrate Virol, Wuhan 430071, Peoples R China.(2001).Purification and characterization of White Spot Syndrome Virus (WSSV) produced in an alternate host: crayfish, Cambarus clarkii.VIRUS RESEARCH,76(2),115-125.
MLA Can-hua Huang,et al."Purification and characterization of White Spot Syndrome Virus (WSSV) produced in an alternate host: crayfish, Cambarus clarkii".VIRUS RESEARCH 76.2(2001):115-125.
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