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Isolation by phage display and characterization of a single-chain antibody specific for O-6-methyldeoxyguanosine
DAI Heping; ZHAO Rouhong; Haug Brenda; Sean M. Hemmingsen; XIAO Wei; Hemmingsen, SM, Natl Res Council Canada, Inst Plant Biotechnol, 110 Gymnasium Rd, Saskatoon, SK S7N 0W9, Canada
2001
Source PublicationCHINESE SCIENCE BULLETIN
Volume46Issue:12Pages:1024-1029
AbstractNew approaches of making single chain Fv antibodies against O-6-methyl-2'-deoxyguanosine (O(6)MdG) have been demonstrated by using the phage antibody display system. Using O(6)MdG as an antigen, 21 positive clones were identified by ELISA from this library, one of which, designated H3, specifically binds to O(6)MdG with high affinity. The H3 scFv antibody has an affinity constant (K-aff) of 5.94 x 10(11)(mol/L)(-1). H3 scFv has been successfully used to detect O-6 MdG in DNA hydrolyses from yeast or E. coli cells treated with a DNA methylating agent. To our knowledge, this is the first report of the selection of a specific scFv against DNA adducts. The results demonstrate the potential applications of the phage display technology for the detection of DNA lesions caused by mutagens and carcinogens.
KeywordO-6-methyl-2 '-deoxyguanosine Phage Display Antibody Single Chain Fv Dna Adducts
DepartmentNatl Res Council Canada, Inst Plant Biotechnol, Saskatoon, SK S7N 0W9, Canada; Chinese Acad Sci, Inst Hydrobiol, Wuhan 430072, Peoples R China; Univ Saskatchewan, Dept Microbiol & Immunol, Saskatoon, SK S7N 5E5, Canada
Subject AreaMultidisciplinary Sciences
Indexed BySCI
Language英语
WOS IDWOS:000170193900014
Citation statistics
Cited Times:4[WOS]   [WOS Record]     [Related Records in WOS]
Document Type期刊论文
Identifierhttp://ir.ihb.ac.cn/handle/152342/9968
Collection期刊论文
Corresponding AuthorHemmingsen, SM, Natl Res Council Canada, Inst Plant Biotechnol, 110 Gymnasium Rd, Saskatoon, SK S7N 0W9, Canada
Recommended Citation
GB/T 7714
DAI Heping,ZHAO Rouhong,Haug Brenda,et al. Isolation by phage display and characterization of a single-chain antibody specific for O-6-methyldeoxyguanosine[J]. CHINESE SCIENCE BULLETIN,2001,46(12):1024-1029.
APA DAI Heping,ZHAO Rouhong,Haug Brenda,Sean M. Hemmingsen,XIAO Wei,&Hemmingsen, SM, Natl Res Council Canada, Inst Plant Biotechnol, 110 Gymnasium Rd, Saskatoon, SK S7N 0W9, Canada.(2001).Isolation by phage display and characterization of a single-chain antibody specific for O-6-methyldeoxyguanosine.CHINESE SCIENCE BULLETIN,46(12),1024-1029.
MLA DAI Heping,et al."Isolation by phage display and characterization of a single-chain antibody specific for O-6-methyldeoxyguanosine".CHINESE SCIENCE BULLETIN 46.12(2001):1024-1029.
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