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学科主题: Multidisciplinary Sciences
题名: Isolation by phage display and characterization of a single-chain antibody specific for O-6-methyldeoxyguanosine
作者: Dai, HP; Zhao, RH; Brenda, H; Hemmingsen, SM; Xiao, W
通讯作者: Hemmingsen, SM, Natl Res Council Canada, Inst Plant Biotechnol, 110 Gymnasium Rd, Saskatoon, SK S7N 0W9, Canada
关键词: O-6-methyl-2 '-deoxyguanosine ; phage display antibody ; single chain Fv ; DNA adducts
刊名: CHINESE SCIENCE BULLETIN
发表日期: 2001-06-01
卷: 46, 期:12, 页:1024-1029
收录类别: SCI
文章类型: Article
部门归属: Natl Res Council Canada, Inst Plant Biotechnol, Saskatoon, SK S7N 0W9, Canada; Chinese Acad Sci, Inst Hydrobiol, Wuhan 430072, Peoples R China; Univ Saskatchewan, Dept Microbiol & Immunol, Saskatoon, SK S7N 5E5, Canada
WOS标题词: Science & Technology
类目[WOS]: Multidisciplinary Sciences
研究领域[WOS]: Science & Technology - Other Topics
摘要: New approaches of making single chain Fv antibodies against O-6-methyl-2'-deoxyguanosine (O(6)MdG) have been demonstrated by using the phage antibody display system. Using O(6)MdG as an antigen, 21 positive clones were identified by ELISA from this library, one of which, designated H3, specifically binds to O(6)MdG with high affinity. The H3 scFv antibody has an affinity constant (K-aff) of 5.94 x 10(11)(mol/L)(-1). H3 scFv has been successfully used to detect O-6 MdG in DNA hydrolyses from yeast or E. coli cells treated with a DNA methylating agent. To our knowledge, this is the first report of the selection of a specific scFv against DNA adducts. The results demonstrate the potential applications of the phage display technology for the detection of DNA lesions caused by mutagens and carcinogens.
英文摘要: New approaches of making single chain Fv antibodies against O-6-methyl-2'-deoxyguanosine (O(6)MdG) have been demonstrated by using the phage antibody display system. Using O(6)MdG as an antigen, 21 positive clones were identified by ELISA from this library, one of which, designated H3, specifically binds to O(6)MdG with high affinity. The H3 scFv antibody has an affinity constant (K-aff) of 5.94 x 10(11)(mol/L)(-1). H3 scFv has been successfully used to detect O-6 MdG in DNA hydrolyses from yeast or E. coli cells treated with a DNA methylating agent. To our knowledge, this is the first report of the selection of a specific scFv against DNA adducts. The results demonstrate the potential applications of the phage display technology for the detection of DNA lesions caused by mutagens and carcinogens.
关键词[WOS]: ESCHERICHIA-COLI ; DNA ; REPAIR ; O-6-METHYLGUANINE ; O6-METHYLGUANINE ; YEAST
语种: 英语
WOS记录号: WOS:000170193900014
ISSN号: 1001-6538
Citation statistics:
内容类型: 期刊论文
URI标识: http://ir.ihb.ac.cn/handle/152342/9968
Appears in Collections:中科院水生所知识产出(2009年前)_期刊论文

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作者单位: 1.Natl Res Council Canada, Inst Plant Biotechnol, Saskatoon, SK S7N 0W9, Canada
2.Chinese Acad Sci, Inst Hydrobiol, Wuhan 430072, Peoples R China
3.Univ Saskatchewan, Dept Microbiol & Immunol, Saskatoon, SK S7N 5E5, Canada

Recommended Citation:
DAI Heping; ZHAO Rouhong; Haug Brenda; Sean M. Hemmingsen; XIAO Wei.Isolation by phage display and characterization of a single-chain antibody specific for O-6-methyldeoxyguanosine,CHINESE SCIENCE BULLETIN,2001,46(12):1024-1029
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