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Molecular characterization and expression of the M6 gene of grass carp hemorrhage virus (GCHV), an aquareovirus - Brief report
T. Qiu; R. H. Lu; J. Zhang and Z. Y. Zhu; Lu, RH, Chinese Acad Sci, Inst Hydrobiol, State Key Lab Freshwater Ecol & Biotechnol, Wuhan 430072, Peoples R China
2001
Source PublicationARCHIVES OF VIROLOGY
Volume146Issue:7Pages:1391-1397
Abstract7The complete nucleotide sequence of M6 gene of grass carp hemorrhage virus (GCHV) was determined. It is 2039 nucleotides in length and contains a single large open reading frame that could encode a protein of 648 amino acids with predicted molecular mass of 68.7 kDa. Amino acid sequence comparison revealed that the protein encoded by GCHV M6 is closely related to the protein mul of mammalian reovirus. The M6 gene, encoding the major outer-capsid protein, was expressed using the pET fusion protein vector in Escherichia coli and detected by Western blotting using chicken anti-GCHV immunoglobulin (IgY). The result indicates that the protein encoded by M6 may share a putative Asn-42-Pro-43 proteolytic cleavage site with mul.
KeywordRna Blot Hybridization Genome Identification Genogroup Sequence Segments Mu-1c
DepartmentChinese Acad Sci, Inst Hydrobiol, State Key Lab Freshwater Ecol & Biotechnol, Wuhan 430072, Peoples R China
Subject AreaVirology
Indexed BySCI
WOS IDWOS:000170368200012
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Document Type期刊论文
Identifierhttp://ir.ihb.ac.cn/handle/152342/9966
Collection期刊论文
Corresponding AuthorLu, RH, Chinese Acad Sci, Inst Hydrobiol, State Key Lab Freshwater Ecol & Biotechnol, Wuhan 430072, Peoples R China
Recommended Citation
GB/T 7714
T. Qiu,R. H. Lu,J. Zhang and Z. Y. Zhu,et al. Molecular characterization and expression of the M6 gene of grass carp hemorrhage virus (GCHV), an aquareovirus - Brief report[J]. ARCHIVES OF VIROLOGY,2001,146(7):1391-1397.
APA T. Qiu,R. H. Lu,J. Zhang and Z. Y. Zhu,&Lu, RH, Chinese Acad Sci, Inst Hydrobiol, State Key Lab Freshwater Ecol & Biotechnol, Wuhan 430072, Peoples R China.(2001).Molecular characterization and expression of the M6 gene of grass carp hemorrhage virus (GCHV), an aquareovirus - Brief report.ARCHIVES OF VIROLOGY,146(7),1391-1397.
MLA T. Qiu,et al."Molecular characterization and expression of the M6 gene of grass carp hemorrhage virus (GCHV), an aquareovirus - Brief report".ARCHIVES OF VIROLOGY 146.7(2001):1391-1397.
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