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Differential gene expression in fully-grown oocytes between gynogenetic and gonochoristic crucian carps
Jing Xie; Jian-Jun Wen; Bo Chen; Jian-Fang Gui; Gui, JF, Chinese Acad Sci, Inst Hydrobiol, State Key Lab Freshwater Ecol & Biotechnol, Wuhan 430072, Peoples R China
2001
Source PublicationGENE
ISSN0378-1119
Volume271Issue:1Pages:109-116
AbstractSilver crucian carp (Carassius auratus gibelio) is a unique triploid bisexual species that can reproduce by gynogenesis. As all other gynogenetic animals, it keeps its chromosome integrity by inhibiting the first meiosis division (no extrusion of the first pole body). To understand the molecular events governing this reproduction mode, suppression subtractive hybridization was used to identify the genes differentially expressed in fully-grown oocytes of the gynogenetic and gonochoristic crucian carp (gyno-carp and gono-carp). From two specific subtractive cDNA libraries, the clones screened out by dot blots and virtual Northern blots were chosen to clone, full-length cDNA by RACE. Four differentially expressed genes were obtained. Two are novel genes and are expressed specifically in the oocytes. The gyno-carp stores much more mRNA of cyclin A2, a new member of the fish A-type cyclin gene, in its fully-grown oocyte than in the gono-carp. The last gene is histone H2A. The histone H2As of these two closely related crucian carps are quite different in the C-terminus. Preliminary characterization of the four genes has been analyzed by nucleotide and deduced amino acid sequence and Northern analysis. (C) 2001 Elsevier Science B.V. All rights reserved.
KeywordDifferential Gene Expression Gynogenesis Oogenesis Oocytes Suppression Subtractive Hybridization
DepartmentChinese Acad Sci, Inst Hydrobiol, State Key Lab Freshwater Ecol & Biotechnol, Wuhan 430072, Peoples R China
Subject AreaGenetics & Heredity
Indexed BySCI
WOS IDWOS:000170329200013
Citation statistics
Document Type期刊论文
Identifierhttp://ir.ihb.ac.cn/handle/152342/9956
Collection期刊论文
Corresponding AuthorGui, JF, Chinese Acad Sci, Inst Hydrobiol, State Key Lab Freshwater Ecol & Biotechnol, Wuhan 430072, Peoples R China
Recommended Citation
GB/T 7714
Jing Xie,Jian-Jun Wen,Bo Chen,et al. Differential gene expression in fully-grown oocytes between gynogenetic and gonochoristic crucian carps[J]. GENE,2001,271(1):109-116.
APA Jing Xie,Jian-Jun Wen,Bo Chen,Jian-Fang Gui,&Gui, JF, Chinese Acad Sci, Inst Hydrobiol, State Key Lab Freshwater Ecol & Biotechnol, Wuhan 430072, Peoples R China.(2001).Differential gene expression in fully-grown oocytes between gynogenetic and gonochoristic crucian carps.GENE,271(1),109-116.
MLA Jing Xie,et al."Differential gene expression in fully-grown oocytes between gynogenetic and gonochoristic crucian carps".GENE 271.1(2001):109-116.
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