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学科主题: Virology
题名: Genome segment S8 of grass carp hemorrhage virus encodes a virion protein
作者: Qiu, T; Zhang, J; Lu, RH; Zhu, ZY
通讯作者: Lu, RH, Chinese Acad Sci, Inst Hydrobiol, IHB,State Key Lab Freshwater Ecol & Biotechnol, Dept Genet & Breeding, Wuhan 430072, Peoples R China
关键词: RNA virus ; GCHV ; segment S8 ; nucleoticle sequence ; virion protein ; IgY
刊名: INTERVIROLOGY
发表日期: 2001-09-01
卷: 44, 期:5, 页:317-320
收录类别: SCI
文章类型: Article
部门归属: Chinese Acad Sci, Inst Hydrobiol, IHB,State Key Lab Freshwater Ecol & Biotechnol, Dept Genet & Breeding, Wuhan 430072, Peoples R China
WOS标题词: Science & Technology ; Life Sciences & Biomedicine
类目[WOS]: Virology
研究领域[WOS]: Virology
摘要: The complete nucleotide sequence of the genome segment S8 of grass carp hemorrhage virus (GCHV) was determined from cDNA corresponding to the viral genomic RNA. It is 1,287 nucleotides in length and contains a large open reading frame that could encode a protein of 409 amino acids with a predicted molecular mass of 44 kD. The S8 was expressed using the pET fusion protein vector and detected by Western blotting analysis using the chicken egg IgY against intact GCHV particles, indicating that S8 encodes a virion protein. Amino acid sequence comparisons revealed that the protein encoded by S8 is closely related to protein alpha2 of mammalian reovirus, suggesting that the deduced protein of S8 is an inner capsid protein. Copyright (C) 2001 S. Karger AG, Basel.
英文摘要: The complete nucleotide sequence of the genome segment S8 of grass carp hemorrhage virus (GCHV) was determined from cDNA corresponding to the viral genomic RNA. It is 1,287 nucleotides in length and contains a large open reading frame that could encode a protein of 409 amino acids with a predicted molecular mass of 44 kD. The S8 was expressed using the pET fusion protein vector and detected by Western blotting analysis using the chicken egg IgY against intact GCHV particles, indicating that S8 encodes a virion protein. Amino acid sequence comparisons revealed that the protein encoded by S8 is closely related to protein alpha2 of mammalian reovirus, suggesting that the deduced protein of S8 is an inner capsid protein. Copyright (C) 2001 S. Karger AG, Basel.
关键词[WOS]: SEQUENCE ; REOVIRUS ; AQUAREOVIRUS ; CLONING
语种: 英语
WOS记录号: WOS:000171984100007
ISSN号: 0300-5526
Citation statistics:
内容类型: 期刊论文
URI标识: http://ir.ihb.ac.cn/handle/152342/9922
Appears in Collections:中科院水生所知识产出(2009年前)_期刊论文

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作者单位: 1.Chinese Acad Sci, Inst Hydrobiol, IHB,State Key Lab Freshwater Ecol & Biotechnol, Dept Genet & Breeding, Wuhan 430072, Peoples R China

Recommended Citation:
Qiu, T; Zhang, J; Lu, RH; Zhu, ZY.Genome segment S8 of grass carp hemorrhage virus encodes a virion protein,INTERVIROLOGY,2001,44(5):317-320
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