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In vitro culture of embryonic hearts from guppy fish (Poecilia reticulata)
ZHANG Qiya; LI Zhengqiu &GUl Jianfang; Zhang, QY, Chinese Acad Sci, Inst Hydrobiol, State Key Lab Freshwater Ecol & Biotechnol, Wuhan 430072, Peoples R China
2001
Source PublicationCHINESE SCIENCE BULLETIN
Volume46Issue:19Pages:1638-+
AbstractForty embryonic hearts were taken out by anatomical needle from denuded embryos of the ovoviviparity guppy fish that were dechorioned by mechanic method or by trypsin digestion, and were in vitro cultured. In the cultured hearts, 80% have maintained beating in vitro for 4 weeks, and the longest record for beating was 142 d. Owing to fish embryo transparency, beating frequency and blood color changes are easily viewed from the embryonic hearts under a dissecting microscope. The current study established the in vitro culture method of embryonic hearts in guppy fish, which can be used as a model for the study of heart and cardiovascular system in vertebrates.
KeywordGuppy Fish Embryo Heart In Vitro Culture
DepartmentChinese Acad Sci, Inst Hydrobiol, State Key Lab Freshwater Ecol & Biotechnol, Wuhan 430072, Peoples R China
Subject AreaMultidisciplinary Sciences
Indexed BySCI
Language英语
WOS IDWOS:000172324000016
Citation statistics
Cited Times:1[WOS]   [WOS Record]     [Related Records in WOS]
Document Type期刊论文
Identifierhttp://ir.ihb.ac.cn/handle/152342/9918
Collection期刊论文
Corresponding AuthorZhang, QY, Chinese Acad Sci, Inst Hydrobiol, State Key Lab Freshwater Ecol & Biotechnol, Wuhan 430072, Peoples R China
Recommended Citation
GB/T 7714
ZHANG Qiya,LI Zhengqiu &GUl Jianfang,Zhang, QY, Chinese Acad Sci, Inst Hydrobiol, State Key Lab Freshwater Ecol & Biotechnol, Wuhan 430072, Peoples R China. In vitro culture of embryonic hearts from guppy fish (Poecilia reticulata)[J]. CHINESE SCIENCE BULLETIN,2001,46(19):1638-+.
APA ZHANG Qiya,LI Zhengqiu &GUl Jianfang,&Zhang, QY, Chinese Acad Sci, Inst Hydrobiol, State Key Lab Freshwater Ecol & Biotechnol, Wuhan 430072, Peoples R China.(2001).In vitro culture of embryonic hearts from guppy fish (Poecilia reticulata).CHINESE SCIENCE BULLETIN,46(19),1638-+.
MLA ZHANG Qiya,et al."In vitro culture of embryonic hearts from guppy fish (Poecilia reticulata)".CHINESE SCIENCE BULLETIN 46.19(2001):1638-+.
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