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学科主题: Multidisciplinary Sciences
题名: In vitro culture of embryonic hearts from guppy fish (Poecilia reticulata)
作者: ZHANG Qiya ; LI Zhengqiu &GUl Jianfang
通讯作者: Zhang, QY, Chinese Acad Sci, Inst Hydrobiol, State Key Lab Freshwater Ecol & Biotechnol, Wuhan 430072, Peoples R China
关键词: guppy fish ; embryo ; heart ; in vitro culture
刊名: CHINESE SCIENCE BULLETIN
发表日期: 2001
卷: 46, 期:19, 页:1638-+
收录类别: SCI
部门归属: Chinese Acad Sci, Inst Hydrobiol, State Key Lab Freshwater Ecol & Biotechnol, Wuhan 430072, Peoples R China
摘要: Forty embryonic hearts were taken out by anatomical needle from denuded embryos of the ovoviviparity guppy fish that were dechorioned by mechanic method or by trypsin digestion, and were in vitro cultured. In the cultured hearts, 80% have maintained beating in vitro for 4 weeks, and the longest record for beating was 142 d. Owing to fish embryo transparency, beating frequency and blood color changes are easily viewed from the embryonic hearts under a dissecting microscope. The current study established the in vitro culture method of embryonic hearts in guppy fish, which can be used as a model for the study of heart and cardiovascular system in vertebrates.
语种: 英语
WOS记录号: WOS:000172324000016
Citation statistics:
内容类型: 期刊论文
URI标识: http://ir.ihb.ac.cn/handle/152342/9918
Appears in Collections:中科院水生所知识产出(2009年前)_期刊论文

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Recommended Citation:
ZHANG Qiya; LI Zhengqiu &GUl Jianfang.In vitro culture of embryonic hearts from guppy fish (Poecilia reticulata),CHINESE SCIENCE BULLETIN,2001,46(19):1638-+
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