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Three-piece-ligation PCR and application in disruption of chlorophyll synthesis genes in Synechocystis sp PCC 6803
Renqiu Kong; Xudong Xu; Xu, XD, Chinese Acad Sci, Inst Hydrobiol, Wuhan 430072, Hubei, Peoples R China
2002
Source PublicationCURRENT MICROBIOLOGY
Volume44Issue:4Pages:241-245
AbstractLinear DNA, consisting of a drug-resistance marker and long flanking sequences, was synthesized by one-step polymerase chain reaction after a three-piece ligating reaction. Chlorophyll synthesis genes, chlH and chIL in Synechocystis sp. PCC 6803, were replaced by a kanamycin-resistance marker through double recombinations with flanking homology regions. Under LAHG conditions, the chIL but not chlH mutant stopped chlorophyll synthesis, while both synthesized chlorophyll in the light.
KeywordEscherichia-coli Biosynthesis Pcc-6803 Pcc6803
DepartmentChinese Acad Sci, Inst Hydrobiol, Wuhan 430072, Hubei, Peoples R China
Subject AreaMicrobiology
Indexed BySCI
WOS IDWOS:000174316400003
Citation statistics
Document Type期刊论文
Identifierhttp://ir.ihb.ac.cn/handle/152342/9876
Collection期刊论文
Corresponding AuthorXu, XD, Chinese Acad Sci, Inst Hydrobiol, Wuhan 430072, Hubei, Peoples R China
Recommended Citation
GB/T 7714
Renqiu Kong,Xudong Xu,Xu, XD, Chinese Acad Sci, Inst Hydrobiol, Wuhan 430072, Hubei, Peoples R China. Three-piece-ligation PCR and application in disruption of chlorophyll synthesis genes in Synechocystis sp PCC 6803[J]. CURRENT MICROBIOLOGY,2002,44(4):241-245.
APA Renqiu Kong,Xudong Xu,&Xu, XD, Chinese Acad Sci, Inst Hydrobiol, Wuhan 430072, Hubei, Peoples R China.(2002).Three-piece-ligation PCR and application in disruption of chlorophyll synthesis genes in Synechocystis sp PCC 6803.CURRENT MICROBIOLOGY,44(4),241-245.
MLA Renqiu Kong,et al."Three-piece-ligation PCR and application in disruption of chlorophyll synthesis genes in Synechocystis sp PCC 6803".CURRENT MICROBIOLOGY 44.4(2002):241-245.
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