中国科学院水生生物研究所机构知识库
Advanced  
IHB OpenIR  > 中科院水生所知识产出(2009年前)  > 期刊论文
学科主题: Biochemistry & Molecular Biology
题名: Structure-function analysis of the inverted terminal repeats of the Sleeping Beauty transposon
作者: Zongbin Cui ; Aron M.Geurts ; Geyi Liu ; Christopher D.Kaufman ; Perry B.Hackett
通讯作者: Hackett, PB, Discovery Genom Inc, 614 McKinley Pl, Minneapolis, MN 55413 USA
关键词: human cells ; gene therapy ; HeLa cells ; vertebrates
刊名: JOURNAL OF MOLECULAR BIOLOGY
发表日期: 2002
卷: 318, 期:5, 页:1221-1235
收录类别: SCI
部门归属: Chinese Acad Sci, Inst Hydrobiol, Wuhan 430072, Peoples R China; Discovery Genom Inc, Minneapolis, MN 55413 USA; Univ Minnesota, Dept Genet Cell Biol & Dev, St Paul, MN 55108 USA; Univ Minnesota, Arnold & Mabel Bechman Ctr Transposon Res, St Paul, MN 55108 USA
摘要: Translocation of Sleeping Beauty (SB) transposon requires specific binding of SB transposase to inverted terminal repeats (ITRs) of about 230 bp at each end of the transposon, which is followed by a cut-and-paste transfer of the transposon into a target DNA sequence. The ITRs contain two imperfect direct repeats (DRs) of about 32 bp. The outer DRs are at the extreme ends of the transposon whereas the inner DRs are located inside the transposon, 165-166 bp from the outer DRs. Here we investigated the roles of the DR elements in transposition. Although there is a core transposase-binding sequence common to all of the DRs, additional adjacent sequences are required for transposition and these sequences vary in the different DRs. As a result, SB transposase binds less tightly to the outer DRs than to the inner DRs. Two DRs are required in each ITR for transposition but they are not interchangeable for efficient transposition. Each DR appears to have a distinctive role in transposition. The spacing and sequence between the DR elements in an ITR affect transposition rates, suggesting a constrained geometry is involved in the interactions of SB transposase molecules in order to achieve precise mobilization. Transposons are flanked by TA dinucleotide base-pairs that are important for excision; elimination of the TA motif on one side of the transposon significantly reduces transposition while loss of TAs on both flanks of the transposon abolishes transposition. These findings have led to the construction of a more advanced transposon that should be useful in gene transfer and insertional mutagenesis in vertebrates. (C) 2002 Elsevier Science Ltd. All rights reserved.
WOS记录号: WOS:000175767900005
Citation statistics:
内容类型: 期刊论文
URI标识: http://ir.ihb.ac.cn/handle/152342/9858
Appears in Collections:中科院水生所知识产出(2009年前)_期刊论文

Files in This Item: Download All
File Name/ File Size Content Type Version Access License
Structure-function analysis of the inverted terminal repeats of the Sleeping Beauty transposon.pdf(765KB)----开放获取--View Download

Recommended Citation:
Zongbin Cui; Aron M.Geurts; Geyi Liu; Christopher D.Kaufman; Perry B.Hackett.Structure-function analysis of the inverted terminal repeats of the Sleeping Beauty transposon,JOURNAL OF MOLECULAR BIOLOGY,2002,318(5):1221-1235
Service
Recommend this item
Sava as my favorate item
Show this item's statistics
Export Endnote File
Google Scholar
Similar articles in Google Scholar
[Zongbin Cui]'s Articles
[Aron M.Geurts]'s Articles
[Geyi Liu]'s Articles
CSDL cross search
Similar articles in CSDL Cross Search
[Zongbin Cui]‘s Articles
[Aron M.Geurts]‘s Articles
[Geyi Liu]‘s Articles
Related Copyright Policies
Null
Social Bookmarking
Add to CiteULike Add to Connotea Add to Del.icio.us Add to Digg Add to Reddit
文件名: Structure-function analysis of the inverted terminal repeats of the Sleeping Beauty transposon.pdf
格式: Adobe PDF
此文件暂不支持浏览
所有评论 (0)
暂无评论
 
评注功能仅针对注册用户开放,请您登录
您对该条目有什么异议,请填写以下表单,管理员会尽快联系您。
内 容:
Email:  *
单位:
验证码:   刷新
您在IR的使用过程中有什么好的想法或者建议可以反馈给我们。
标 题:
 *
内 容:
Email:  *
验证码:   刷新

Items in IR are protected by copyright, with all rights reserved, unless otherwise indicated.

 

 

Valid XHTML 1.0!
Copyright © 2007-2016  中国科学院水生生物研究所 - Feedback
Powered by CSpace