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学科主题: Fisheries; Marine & Freshwater Biology
题名: Development of polymorphic EST markers in Penaeus monodon: applications in penaeid genetics
作者: J. Tong ; S.A. Lehnert ; K. Byrne ; H.S. Kwan ; K.H. Chu
通讯作者: Chu, KH, Chinese Univ Hong Kong, Dept Biol, Shatin, Hong Kong, Peoples R China
关键词: expressed sequence tags ; Penaeus monodon ; genome mapping ; shrimp
刊名: AQUACULTURE
发表日期: 2002
卷: 208, 期:1-2, 页:69-79
收录类别: SCI
部门归属: Chinese Univ Hong Kong, Dept Biol, Shatin, Hong Kong, Peoples R China; Chinese Acad Sci, Inst Hydrobiol, Wuhan 430072, Hubei, Peoples R China; CSIRO, Mol Anim Genet ctr, Livestock Ind, St Lucia, Qld 4072, Australia
摘要: The objective of this study was to develop type I markers for genome mapping and other genetic studies of Penaeus monodon. Primers were designed based on expressed sequence tags (ESTs) from a P monodon cephalothorax cDNA library to amplify 100-300 bp products. 34 of the primer pairs successfully amplified PCR products from genomic DNA. Single-strand conformation polymorphism analysis showed that similar to 30% of the ESTs tested exhibit polymorphism in a test panel of P monodon individuals. Mendelian inheritance of the EST-derived markers has been established in two international reference mapping families of P monodon, and mapping of these markers is in progress. Some ESTs were successfully amplified from other Penaeus species (P. chinensis, P japonicus and P vannamei), indicating that the markers are applicable in cross-species comparison. Two populations of P. japonicus could be differentiated using one of the ESTS. In conclusion, the polymorphic EST markers developed in this study are applicable in genome mapping and population genetic studies of penaeid shrimp. (C) 2002 Elsevier Science B.V. All rights reserved.
WOS记录号: WOS:000176129000007
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内容类型: 期刊论文
URI标识: http://ir.ihb.ac.cn/handle/152342/9842
Appears in Collections:中科院水生所知识产出(2009年前)_期刊论文

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J. Tong; S.A. Lehnert; K. Byrne; H.S. Kwan; K.H. Chu.Development of polymorphic EST markers in Penaeus monodon: applications in penaeid genetics,AQUACULTURE,2002,208(1-2):69-79
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