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Development of polymorphic EST markers in Penaeus monodon: applications in penaeid genetics
J. Tong; S.A. Lehnert; K. Byrne; H.S. Kwan; K.H. Chu; Chu, KH, Chinese Univ Hong Kong, Dept Biol, Shatin, Hong Kong, Peoples R China
2002
Source PublicationAQUACULTURE
Volume208Issue:1-2Pages:69-79
AbstractThe objective of this study was to develop type I markers for genome mapping and other genetic studies of Penaeus monodon. Primers were designed based on expressed sequence tags (ESTs) from a P monodon cephalothorax cDNA library to amplify 100-300 bp products. 34 of the primer pairs successfully amplified PCR products from genomic DNA. Single-strand conformation polymorphism analysis showed that similar to 30% of the ESTs tested exhibit polymorphism in a test panel of P monodon individuals. Mendelian inheritance of the EST-derived markers has been established in two international reference mapping families of P monodon, and mapping of these markers is in progress. Some ESTs were successfully amplified from other Penaeus species (P. chinensis, P japonicus and P vannamei), indicating that the markers are applicable in cross-species comparison. Two populations of P. japonicus could be differentiated using one of the ESTS. In conclusion, the polymorphic EST markers developed in this study are applicable in genome mapping and population genetic studies of penaeid shrimp. (C) 2002 Elsevier Science B.V. All rights reserved.
KeywordExpressed Sequence Tags Penaeus Monodon Genome Mapping Shrimp
DepartmentChinese Univ Hong Kong, Dept Biol, Shatin, Hong Kong, Peoples R China; Chinese Acad Sci, Inst Hydrobiol, Wuhan 430072, Hubei, Peoples R China; CSIRO, Mol Anim Genet ctr, Livestock Ind, St Lucia, Qld 4072, Australia
Subject AreaFisheries ; Marine & Freshwater Biology
Indexed BySCI
WOS IDWOS:000176129000007
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Document Type期刊论文
Identifierhttp://ir.ihb.ac.cn/handle/152342/9842
Collection期刊论文
Corresponding AuthorChu, KH, Chinese Univ Hong Kong, Dept Biol, Shatin, Hong Kong, Peoples R China
Recommended Citation
GB/T 7714
J. Tong,S.A. Lehnert,K. Byrne,et al. Development of polymorphic EST markers in Penaeus monodon: applications in penaeid genetics[J]. AQUACULTURE,2002,208(1-2):69-79.
APA J. Tong,S.A. Lehnert,K. Byrne,H.S. Kwan,K.H. Chu,&Chu, KH, Chinese Univ Hong Kong, Dept Biol, Shatin, Hong Kong, Peoples R China.(2002).Development of polymorphic EST markers in Penaeus monodon: applications in penaeid genetics.AQUACULTURE,208(1-2),69-79.
MLA J. Tong,et al."Development of polymorphic EST markers in Penaeus monodon: applications in penaeid genetics".AQUACULTURE 208.1-2(2002):69-79.
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