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Screen for stage-specific expression genes between tail bud stage and heartbeat beginning stage in embryogenesis of gynogenetic silver crucian carp
YAO HUA SHI; JUN LIU; JIAN HONG XIA; JIAN FANG GUI; Gui, JF, Chinese Acad Sci, State Key Lab Freshwater Ecol & Biotechnol, Wuhan Ctr Dev Biol, Inst Hydrobiol, Wuhan 430072, Peoples R China
2002
Source PublicationCELL RESEARCH
Volume12Issue:2Pages:133-142
AbstractA systemic study was initiated to identify stage-specific expression genes in fish embryogenesis by using suppression subtractive hybridization (SSH) technique. In this study, we presented a preliminary result on screen for stage-specific expression genes between tail bud stage (TBS) and heartbeat beginning stage (HBS) in gynogenetic silver crucian carp (Carassius auratus gibelio). Two SSH plasmid libraries specific for TBS embryos and HBS embryos were constructed, and stage-specific expression genes were screened between the two stages. 1963 TBS positive clones and 2466 HBS positive clones were sampled to PCR amplification, and 1373 TBS and 1809 HBS PCR positive clones were selected to carry out dot blots. 169 TBS dot blot positive clones and 272 HBS dot blot positive clones were sequenced. Searching GenBank by using these nucleotide sequences indicated that most of the TBS dot blot positive clones could not be found homologous sequences in the database, while known genes were mainly detected from HBS dot blot positive clones. Of the 79 known genes, 20 were enzymes or kinases involved in important metabolism of embryonic development. Moreover, specific expressions of partial genes were further confirmed by virtual northern blots. This study is the first step for making a large attempt to study temporal and spatial control of gene expression in the gynogenetic fish embryogenesis.
KeywordSsh Embryonic Development Differential Expression Genes Gynogenesis Silver Crucian Carp
DepartmentChinese Acad Sci, State Key Lab Freshwater Ecol & Biotechnol, Wuhan Ctr Dev Biol, Inst Hydrobiol, Wuhan 430072, Peoples R China
Subject AreaCell Biology
Indexed BySCI
WOS IDWOS:000176627300006
Citation statistics
Cited Times:14[WOS]   [WOS Record]     [Related Records in WOS]
Document Type期刊论文
Identifierhttp://ir.ihb.ac.cn/handle/152342/9832
Collection期刊论文
Corresponding AuthorGui, JF, Chinese Acad Sci, State Key Lab Freshwater Ecol & Biotechnol, Wuhan Ctr Dev Biol, Inst Hydrobiol, Wuhan 430072, Peoples R China
Recommended Citation
GB/T 7714
YAO HUA SHI,JUN LIU,JIAN HONG XIA,et al. Screen for stage-specific expression genes between tail bud stage and heartbeat beginning stage in embryogenesis of gynogenetic silver crucian carp[J]. CELL RESEARCH,2002,12(2):133-142.
APA YAO HUA SHI,JUN LIU,JIAN HONG XIA,JIAN FANG GUI,&Gui, JF, Chinese Acad Sci, State Key Lab Freshwater Ecol & Biotechnol, Wuhan Ctr Dev Biol, Inst Hydrobiol, Wuhan 430072, Peoples R China.(2002).Screen for stage-specific expression genes between tail bud stage and heartbeat beginning stage in embryogenesis of gynogenetic silver crucian carp.CELL RESEARCH,12(2),133-142.
MLA YAO HUA SHI,et al."Screen for stage-specific expression genes between tail bud stage and heartbeat beginning stage in embryogenesis of gynogenetic silver crucian carp".CELL RESEARCH 12.2(2002):133-142.
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