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学科主题: Multidisciplinary Sciences
题名: Nuclear transplantation in different strains of zebrafish
作者: HU Wei ; WANG Yaping ; CHEN Shangping ; ZHU Zuoyan
通讯作者: Zhu, ZY, Chinese Acad Sci, Inst Hydrobiol, State Key Lab Freshwater Ecol & Biotechnol, Wuhan 430072, Peoples R China
关键词: zebrafish ; different strains ; nuclear transplantation ; DNA fingerprint
刊名: CHINESE SCIENCE BULLETIN
发表日期: 2002
卷: 47, 期:15, 页:1277-1280
收录类别: SCI
部门归属: Chinese Acad Sci, Inst Hydrobiol, State Key Lab Freshwater Ecol & Biotechnol, Wuhan 430072, Peoples R China
摘要: Single later blastula nuclei from AB strain of zebrafish (Danio rerio) were transplanted into enucleated unfertilized eggs of Long fin strain. Of 1119 cloning embryos, 14 reconstructed embryos developed into fry. DNA fingerprinting systems of the cloned fish were similar to those of the nuclear donor fish, but were distinctly different from those of the unclear recipient fish. It confirmed that the genetic material originated from nuclear donor cell other than from nuclear recipient egg. The research suggested that the basic technique for nuclear transplantation performed with different strains of zebrafish has made a breakthrough. It should be helpful for the study of some important developmental problems such as gene function, the regulation of gene expression during animal development, the developmental potential of a nucleus and the interactions between the donor nucleus and the recipient cytoplasm, etc.
WOS记录号: WOS:000177045600009
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内容类型: 期刊论文
URI标识: http://ir.ihb.ac.cn/handle/152342/9826
Appears in Collections:中科院水生所知识产出(2009年前)_期刊论文

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HU Wei; WANG Yaping; CHEN Shangping; ZHU Zuoyan.Nuclear transplantation in different strains of zebrafish,CHINESE SCIENCE BULLETIN,2002,47(15):1277-1280
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