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Nuclear transplantation in different strains of zebrafish
HU Wei; WANG Yaping; CHEN Shangping; ZHU Zuoyan; Zhu, ZY, Chinese Acad Sci, Inst Hydrobiol, State Key Lab Freshwater Ecol & Biotechnol, Wuhan 430072, Peoples R China
2002
Source PublicationCHINESE SCIENCE BULLETIN
Volume47Issue:15Pages:1277-1280
AbstractSingle later blastula nuclei from AB strain of zebrafish (Danio rerio) were transplanted into enucleated unfertilized eggs of Long fin strain. Of 1119 cloning embryos, 14 reconstructed embryos developed into fry. DNA fingerprinting systems of the cloned fish were similar to those of the nuclear donor fish, but were distinctly different from those of the unclear recipient fish. It confirmed that the genetic material originated from nuclear donor cell other than from nuclear recipient egg. The research suggested that the basic technique for nuclear transplantation performed with different strains of zebrafish has made a breakthrough. It should be helpful for the study of some important developmental problems such as gene function, the regulation of gene expression during animal development, the developmental potential of a nucleus and the interactions between the donor nucleus and the recipient cytoplasm, etc.
KeywordZebrafish Different Strains Nuclear Transplantation Dna Fingerprint
DepartmentChinese Acad Sci, Inst Hydrobiol, State Key Lab Freshwater Ecol & Biotechnol, Wuhan 430072, Peoples R China
Subject AreaMultidisciplinary Sciences
Indexed BySCI
WOS IDWOS:000177045600009
Citation statistics
Cited Times:7[WOS]   [WOS Record]     [Related Records in WOS]
Document Type期刊论文
Identifierhttp://ir.ihb.ac.cn/handle/152342/9826
Collection期刊论文
Corresponding AuthorZhu, ZY, Chinese Acad Sci, Inst Hydrobiol, State Key Lab Freshwater Ecol & Biotechnol, Wuhan 430072, Peoples R China
Recommended Citation
GB/T 7714
HU Wei,WANG Yaping,CHEN Shangping,et al. Nuclear transplantation in different strains of zebrafish[J]. CHINESE SCIENCE BULLETIN,2002,47(15):1277-1280.
APA HU Wei,WANG Yaping,CHEN Shangping,ZHU Zuoyan,&Zhu, ZY, Chinese Acad Sci, Inst Hydrobiol, State Key Lab Freshwater Ecol & Biotechnol, Wuhan 430072, Peoples R China.(2002).Nuclear transplantation in different strains of zebrafish.CHINESE SCIENCE BULLETIN,47(15),1277-1280.
MLA HU Wei,et al."Nuclear transplantation in different strains of zebrafish".CHINESE SCIENCE BULLETIN 47.15(2002):1277-1280.
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