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学科主题: Biochemistry & Molecular Biology; Fisheries; Physiology
题名: Effects of cell-cycle arrest agents on cleavage and development of loach (Misgurnus anguillicaudatus) embryos
作者: T.M. Liu ; C.J. Wu ; X.M. Yu ; X.Y. Li
通讯作者: Wu, CJ, Chinese Acad Sci, State Key Lab Freshwater Ecol & Biotechnol, Inst Hydrobiol, Wuhan 430072, Hubei, Peoples R China
关键词: cell cycle arrest ; colchicine ; loach ; nocodazole ; reversibility ; synchronization ; toxicity
刊名: FISH PHYSIOLOGY AND BIOCHEMISTRY
发表日期: 2001
卷: 25, 期:1, 页:3-9
收录类别: SCI
部门归属: Chinese Acad Sci, State Key Lab Freshwater Ecol & Biotechnol, Inst Hydrobiol, Wuhan 430072, Hubei, Peoples R China
摘要: The aim of this study was to determine the lowest concentration of nocodazole and colchicine to arrest blastomere division during the cleavage stage of loach embryos and to assess the reversibility and toxicity of the treatments in the treated embryos. Eight-cell loach embryos were incubated for 4, 8, 12, or 16 h in 1/10x Holtfreter supplemented with either nocodazole, an inhibitor of tubulin polymerization, or colchicine, an inhibitor of tubulin assembly. Complete arrest of cell cycle was observed, at a colchicine concentration of 0.996 mM and at a nocodazole concentration of 0.275 muM, respectively (the lowest effective concentration). No major morphological alteration in chromatin was observed. Reversibility and toxicity of both agents were dose and exposure period dependent. For both agents, prolonging cleavage arrest for more than 4 h (at the effective concentrations) is detrimental to development of embryos. Nocodazole treatment was less cytotoxic, whereas the concentrations of colchicine which induce cleavage arrest were detrimental to development beyond the blastula stage. Toxic effects beyond the blastula stage could be minimized for both agents by reducing the period of treatment and concentration.
WOS记录号: WOS:000177379800001
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内容类型: 期刊论文
URI标识: http://ir.ihb.ac.cn/handle/152342/9818
Appears in Collections:中科院水生所知识产出(2009年前)_期刊论文

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Recommended Citation:
T.M. Liu; C.J. Wu; X.M. Yu; X.Y. Li.Effects of cell-cycle arrest agents on cleavage and development of loach (Misgurnus anguillicaudatus) embryos,FISH PHYSIOLOGY AND BIOCHEMISTRY,2001,25(1):3-9
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