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Factors affecting the efficiency of somatic cell nuclear transplantation in the fish embryo
TONG MING LIU; XIAO MU YU; YU ZHEN YE; JIAN FENG ZHOU; ZHONG WEI WANG; JING GOU TONG and CHING JIANG WU; Wu, CJ, Chinese Acad Sci, State Key Lab Freshwater Ecol & Biotechnol, Inst Hydrobiol, Wuhan 430072, Peoples R China
2002
Source PublicationJOURNAL OF EXPERIMENTAL ZOOLOGY
Volume293Issue:7Pages:719-725
AbstractProcedures to improve somatic cell nuclear transplantation in fish were evaluated. We reported effects of nonirradiated recipient eggs, inactivated recipient eggs, different combinations between recipient eggs and donor cells, duration of serum starvation, generation number, and passage number of donor cells on developmental rates of nuclear transplant (NT) embryos. Exposure to 25,000 R of gamma-rays inactivated recipient eggs. Single nucleus of cultured, synchronized somatic cell from gynogenetic bighead carp (Aristichthys nobilis) was transplanted into nonirradiated or genetically inactivated unfertilized egg of gibel carp (Carassius auratus gibelio). There was no significant difference in developmental rate between nonirradiated and inactivated recipient eggs (27.27% vs. 25.71%, respectively). Chromosome count showed that 70.59% of NT embryos contained 48 chromosomes. It showed that most NT embryos came from donor nuclei of bighead carp, which was supported by microsatellite analysis of NT embryos. But 23.53% of NT embryos contained more than 48 chromosomes. It was presumed that those superfluous chromosomes came from nonirradiated recipient eggs. Besides, 5.88% of NT embryos were chimeras. Eggs of blunt-snout bream (Megalobrama amblycephala) and gibel carp were better recipient eggs than those of loach (Misgurnus anguillicaudatus) (25% and 18.03% vs. 8.43%). Among different duration of serum starvation, developmental rate of NT embryos from somatic nuclei of three-day serum starvation was the highest, reaching 25.71% compared to 14.14% (control), 20% (five-day), and 21.95% (seven-day). Cultured donor cells of less passage facilitated reprogramming of NT embryos than those of more passage. Recloning might improve the developmental rate of NT embryos from the differentiated donor nuclei. Developmental rate of fourth generation was the highest (54.83%) and the lowest for first generation (14.14%) compared to second generation (38.96%) and third generation (53.01%). (C) 2002 Wiley-Liss, Inc.
KeywordEnucleated Oocytes Fetal Fibroblasts Wild-type Cloning Cycle Adult Mice
DepartmentChinese Acad Sci, State Key Lab Freshwater Ecol & Biotechnol, Inst Hydrobiol, Wuhan 430072, Peoples R China
Subject AreaZoology
Indexed BySCI
WOS IDWOS:000179071500009
Citation statistics
Cited Times:11[WOS]   [WOS Record]     [Related Records in WOS]
Document Type期刊论文
Identifierhttp://ir.ihb.ac.cn/handle/152342/9778
Collection期刊论文
Corresponding AuthorWu, CJ, Chinese Acad Sci, State Key Lab Freshwater Ecol & Biotechnol, Inst Hydrobiol, Wuhan 430072, Peoples R China
Recommended Citation
GB/T 7714
TONG MING LIU,XIAO MU YU,YU ZHEN YE,et al. Factors affecting the efficiency of somatic cell nuclear transplantation in the fish embryo[J]. JOURNAL OF EXPERIMENTAL ZOOLOGY,2002,293(7):719-725.
APA TONG MING LIU.,XIAO MU YU.,YU ZHEN YE.,JIAN FENG ZHOU.,ZHONG WEI WANG.,...&Wu, CJ, Chinese Acad Sci, State Key Lab Freshwater Ecol & Biotechnol, Inst Hydrobiol, Wuhan 430072, Peoples R China.(2002).Factors affecting the efficiency of somatic cell nuclear transplantation in the fish embryo.JOURNAL OF EXPERIMENTAL ZOOLOGY,293(7),719-725.
MLA TONG MING LIU,et al."Factors affecting the efficiency of somatic cell nuclear transplantation in the fish embryo".JOURNAL OF EXPERIMENTAL ZOOLOGY 293.7(2002):719-725.
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