中国科学院水生生物研究所机构知识库
Advanced  
IHB OpenIR  > 中科院水生所知识产出(2009年前)  > 期刊论文
学科主题: Zoology
题名: Factors affecting the efficiency of somatic cell nuclear transplantation in the fish embryo
作者: TONG MING LIU ; XIAO MU YU ; YU ZHEN YE ; JIAN FENG ZHOU ; ZHONG WEI WANG ; JING GOU TONG and CHING JIANG WU
通讯作者: Wu, CJ, Chinese Acad Sci, State Key Lab Freshwater Ecol & Biotechnol, Inst Hydrobiol, Wuhan 430072, Peoples R China
关键词: ENUCLEATED OOCYTES ; FETAL FIBROBLASTS ; WILD-TYPE ; CLONING ; CYCLE ; ADULT ; MICE
刊名: JOURNAL OF EXPERIMENTAL ZOOLOGY
发表日期: 2002
卷: 293, 期:7, 页:719-725
收录类别: SCI
部门归属: Chinese Acad Sci, State Key Lab Freshwater Ecol & Biotechnol, Inst Hydrobiol, Wuhan 430072, Peoples R China
摘要: Procedures to improve somatic cell nuclear transplantation in fish were evaluated. We reported effects of nonirradiated recipient eggs, inactivated recipient eggs, different combinations between recipient eggs and donor cells, duration of serum starvation, generation number, and passage number of donor cells on developmental rates of nuclear transplant (NT) embryos. Exposure to 25,000 R of gamma-rays inactivated recipient eggs. Single nucleus of cultured, synchronized somatic cell from gynogenetic bighead carp (Aristichthys nobilis) was transplanted into nonirradiated or genetically inactivated unfertilized egg of gibel carp (Carassius auratus gibelio). There was no significant difference in developmental rate between nonirradiated and inactivated recipient eggs (27.27% vs. 25.71%, respectively). Chromosome count showed that 70.59% of NT embryos contained 48 chromosomes. It showed that most NT embryos came from donor nuclei of bighead carp, which was supported by microsatellite analysis of NT embryos. But 23.53% of NT embryos contained more than 48 chromosomes. It was presumed that those superfluous chromosomes came from nonirradiated recipient eggs. Besides, 5.88% of NT embryos were chimeras. Eggs of blunt-snout bream (Megalobrama amblycephala) and gibel carp were better recipient eggs than those of loach (Misgurnus anguillicaudatus) (25% and 18.03% vs. 8.43%). Among different duration of serum starvation, developmental rate of NT embryos from somatic nuclei of three-day serum starvation was the highest, reaching 25.71% compared to 14.14% (control), 20% (five-day), and 21.95% (seven-day). Cultured donor cells of less passage facilitated reprogramming of NT embryos than those of more passage. Recloning might improve the developmental rate of NT embryos from the differentiated donor nuclei. Developmental rate of fourth generation was the highest (54.83%) and the lowest for first generation (14.14%) compared to second generation (38.96%) and third generation (53.01%). (C) 2002 Wiley-Liss, Inc.
WOS记录号: WOS:000179071500009
Citation statistics:
内容类型: 期刊论文
URI标识: http://ir.ihb.ac.cn/handle/152342/9778
Appears in Collections:中科院水生所知识产出(2009年前)_期刊论文

Files in This Item: Download All
File Name/ File Size Content Type Version Access License
Factors affecting the efficiency of somatic cell nuclear transplantation in the fish embryo.pdf(136KB)----开放获取--View Download

Recommended Citation:
TONG MING LIU; XIAO MU YU; YU ZHEN YE; JIAN FENG ZHOU; ZHONG WEI WANG; JING GOU TONG and CHING JIANG WU.Factors affecting the efficiency of somatic cell nuclear transplantation in the fish embryo,JOURNAL OF EXPERIMENTAL ZOOLOGY,2002,293(7):719-725
Service
Recommend this item
Sava as my favorate item
Show this item's statistics
Export Endnote File
Google Scholar
Similar articles in Google Scholar
[TONG MING LIU]'s Articles
[XIAO MU YU]'s Articles
[YU ZHEN YE]'s Articles
CSDL cross search
Similar articles in CSDL Cross Search
[TONG MING LIU]‘s Articles
[XIAO MU YU]‘s Articles
[YU ZHEN YE]‘s Articles
Related Copyright Policies
Null
Social Bookmarking
Add to CiteULike Add to Connotea Add to Del.icio.us Add to Digg Add to Reddit
文件名: Factors affecting the efficiency of somatic cell nuclear transplantation in the fish embryo.pdf
格式: Adobe PDF
此文件暂不支持浏览
所有评论 (0)
暂无评论
 
评注功能仅针对注册用户开放,请您登录
您对该条目有什么异议,请填写以下表单,管理员会尽快联系您。
内 容:
Email:  *
单位:
验证码:   刷新
您在IR的使用过程中有什么好的想法或者建议可以反馈给我们。
标 题:
 *
内 容:
Email:  *
验证码:   刷新

Items in IR are protected by copyright, with all rights reserved, unless otherwise indicated.

 

 

Valid XHTML 1.0!
Copyright © 2007-2016  中国科学院水生生物研究所 - Feedback
Powered by CSpace