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Detection of hepatotoxic Microcystis strains by PCR with intact cells from both culture and environmental samples
Pan, H; Song, LR; Liu, YD; Borner, T; Song, LR, Chinese Acad Sci, Inst Hydrobiol, Dept Phycol, Loujiashan, Wuhan 430072, Peoples R China
2002
Source PublicationARCHIVES OF MICROBIOLOGY
Volume178Issue:6Pages:421-427
AbstractMicrocystins are small hepatotoxic peptides produced by a number of cyanobacteria. They are synthesized non-ribosomally by multifunctional enzyme complex synthetases encoded by the mcy genes. Primers deduced from mcy genes were designed to discriminate between toxic microcystin-producing strains and non-toxic strains. Thus, PCR-mediated detection of mcy genes could be a simple and efficient means to identify potentially harmful genotypes among cyanobacterial populations in bodies of water. We surveyed the distribution of the mcyB gene in different Microcystis strains isolated from Chinese bodies of water and confirmed that PCR can be reliably used to identify toxic strains. By omitting any DNA purification steps, the modified PCR protocol can greatly simplify the process. Cyanobacterial cells enriched from cultures, field samples, or even sediment samples could be used in the PCR assay. This method proved sensitive enough to detect mcyB genes in samples with less than 2,000 Microcystis cells per ml. Its accuracy, specificity and applicability were confirmed by sequencing selected DNA amplicons, as well as by HPLC, ELISA and mouse bioassay as controls for toxin production of every strain used.
KeywordCyanobacteria Microcystis Microcystin Toxin Peptide Synthetase Whole-cell Pcr
DepartmentChinese Acad Sci, Inst Hydrobiol, Dept Phycol, Wuhan 430072, Peoples R China; Humboldt Univ, Inst Biol, D-10115 Berlin, Germany
Subject AreaMicrobiology
Indexed BySCI
WOS IDWOS:000179674200006
Citation statistics
Cited Times:52[WOS]   [WOS Record]     [Related Records in WOS]
Document Type期刊论文
Identifierhttp://ir.ihb.ac.cn/handle/152342/9766
Collection期刊论文
Corresponding AuthorSong, LR, Chinese Acad Sci, Inst Hydrobiol, Dept Phycol, Loujiashan, Wuhan 430072, Peoples R China
Recommended Citation
GB/T 7714
Pan, H,Song, LR,Liu, YD,et al. Detection of hepatotoxic Microcystis strains by PCR with intact cells from both culture and environmental samples[J]. ARCHIVES OF MICROBIOLOGY,2002,178(6):421-427.
APA Pan, H,Song, LR,Liu, YD,Borner, T,&Song, LR, Chinese Acad Sci, Inst Hydrobiol, Dept Phycol, Loujiashan, Wuhan 430072, Peoples R China.(2002).Detection of hepatotoxic Microcystis strains by PCR with intact cells from both culture and environmental samples.ARCHIVES OF MICROBIOLOGY,178(6),421-427.
MLA Pan, H,et al."Detection of hepatotoxic Microcystis strains by PCR with intact cells from both culture and environmental samples".ARCHIVES OF MICROBIOLOGY 178.6(2002):421-427.
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