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Determination of some reactive oxygen species scavengers based on the peroxidase-oxidase oscillator
Gan, NQ; Cai, RX; Lin, ZX; Song, LR; Cai, RX, Chinese Acad Sci, Inst Hydrobiol, Wuhan 430072, Peoples R China
2003
Source PublicationANALYST
Volume128Issue:3Pages:301-305
AbstractThis paper reports a new method for detection of ROS scavengers including superoxide dismutase, ascorbic acid and glutathione based on a 'probe' of peroxidase-oxidase biochemical oscillator. The oscillation period and amplitude change with different concentrations of scavengers. The linear ranges of superoxide dismutase, ascorbic acid and glutathione are respectively 1.56 x 10(-4)-1.56 x 10(-3) mg mL(-1), 1.75 x 10(-7) -1.75 x 10(-5) mol L-1 and 9.38 x 10(-7) -7.5 x 10(-5) mol L-1. The selectivity, linearity and precision for superoxide dismutase, ascorbic acid, and glutathione are presented and discussed. The results compared well with other standard methods for determination of superoxide dismutase, ascorbic acid and glutathione. Some possible steps in the overall reaction mechanisms are discussed.
KeywordMechanism
DepartmentChinese Acad Sci, Inst Hydrobiol, Wuhan 430072, Peoples R China; Wuhan Univ, Dept Chem, Wuhan 430072, Peoples R China
Subject AreaChemistry ; Analytical
Indexed BySCI
WOS IDWOS:000181357900017
Citation statistics
Cited Times:3[WOS]   [WOS Record]     [Related Records in WOS]
Document Type期刊论文
Identifierhttp://ir.ihb.ac.cn/handle/152342/9740
Collection期刊论文
Corresponding AuthorCai, RX, Chinese Acad Sci, Inst Hydrobiol, Wuhan 430072, Peoples R China
Recommended Citation
GB/T 7714
Gan, NQ,Cai, RX,Lin, ZX,et al. Determination of some reactive oxygen species scavengers based on the peroxidase-oxidase oscillator[J]. ANALYST,2003,128(3):301-305.
APA Gan, NQ,Cai, RX,Lin, ZX,Song, LR,&Cai, RX, Chinese Acad Sci, Inst Hydrobiol, Wuhan 430072, Peoples R China.(2003).Determination of some reactive oxygen species scavengers based on the peroxidase-oxidase oscillator.ANALYST,128(3),301-305.
MLA Gan, NQ,et al."Determination of some reactive oxygen species scavengers based on the peroxidase-oxidase oscillator".ANALYST 128.3(2003):301-305.
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