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学科主题: Chemistry ; Analytical
题名: Determination of some reactive oxygen species scavengers based on the peroxidase-oxidase oscillator
作者: Gan, NQ; Cai, RX; Lin, ZX; Song, LR
通讯作者: Cai, RX, Chinese Acad Sci, Inst Hydrobiol, Wuhan 430072, Peoples R China
关键词: MECHANISM
刊名: ANALYST
发表日期: 2003
DOI: 10.1039/b209502e
卷: 128, 期:3, 页:301-305
收录类别: SCI
文章类型: Article
部门归属: Chinese Acad Sci, Inst Hydrobiol, Wuhan 430072, Peoples R China; Wuhan Univ, Dept Chem, Wuhan 430072, Peoples R China
WOS标题词: Science & Technology ; Physical Sciences
类目[WOS]: Chemistry, Analytical
研究领域[WOS]: Chemistry
摘要: This paper reports a new method for detection of ROS scavengers including superoxide dismutase, ascorbic acid and glutathione based on a 'probe' of peroxidase-oxidase biochemical oscillator. The oscillation period and amplitude change with different concentrations of scavengers. The linear ranges of superoxide dismutase, ascorbic acid and glutathione are respectively 1.56 x 10(-4)-1.56 x 10(-3) mg mL(-1), 1.75 x 10(-7) -1.75 x 10(-5) mol L-1 and 9.38 x 10(-7) -7.5 x 10(-5) mol L-1. The selectivity, linearity and precision for superoxide dismutase, ascorbic acid, and glutathione are presented and discussed. The results compared well with other standard methods for determination of superoxide dismutase, ascorbic acid and glutathione. Some possible steps in the overall reaction mechanisms are discussed.
英文摘要: This paper reports a new method for detection of ROS scavengers including superoxide dismutase, ascorbic acid and glutathione based on a 'probe' of peroxidase-oxidase biochemical oscillator. The oscillation period and amplitude change with different concentrations of scavengers. The linear ranges of superoxide dismutase, ascorbic acid and glutathione are respectively 1.56 x 10(-4)-1.56 x 10(-3) mg mL(-1), 1.75 x 10(-7) -1.75 x 10(-5) mol L-1 and 9.38 x 10(-7) -7.5 x 10(-5) mol L-1. The selectivity, linearity and precision for superoxide dismutase, ascorbic acid, and glutathione are presented and discussed. The results compared well with other standard methods for determination of superoxide dismutase, ascorbic acid and glutathione. Some possible steps in the overall reaction mechanisms are discussed.
关键词[WOS]: MECHANISM ; CHAOS
语种: 英语
WOS记录号: WOS:000181357900017
ISSN号: 0003-2654
Citation statistics:
内容类型: 期刊论文
URI标识: http://ir.ihb.ac.cn/handle/152342/9740
Appears in Collections:中科院水生所知识产出(2009年前)_期刊论文

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作者单位: 1.Chinese Acad Sci, Inst Hydrobiol, Wuhan 430072, Peoples R China
2.Wuhan Univ, Dept Chem, Wuhan 430072, Peoples R China

Recommended Citation:
Gan, NQ; Cai, RX; Lin, ZX; Song, LR.Determination of some reactive oxygen species scavengers based on the peroxidase-oxidase oscillator,ANALYST,2003,128(3):301-305
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