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学科主题: Multidisciplinary Sciences
题名: PCR-RFLP analysis of mitochondrial DNA ND5/6 region among 3 subspecies of common carp (Cyprinus carpio L.) and its application to genetic discrimination of subspecies
作者: Zhou, JF ; Wang, ZW ; Ye, YZ ; Wu, QJ
通讯作者: Wu, QJ, Chinese Acad Sci, Inst Hydrobiol, State Key Lab Freshwater Ecol & Biotechnol, Wuhan 430072, Peoples R China
关键词: Cyprinus carpio ; subspecies ; ND5/6 ; PCR-RFLP ; molecular marker
刊名: CHINESE SCIENCE BULLETIN
发表日期: 2003
卷: 48, 期:5, 页:465-468
收录类别: SCI
部门归属: Chinese Acad Sci, Inst Hydrobiol, State Key Lab Freshwater Ecol & Biotechnol, Wuhan 430072, Peoples R China
摘要: Mitochondrial DNA ND5/6 region was studied by PCR-RFLP analysis among ten representative strains belonging to three subspecies (Cyprinus carpio carpio, Cyprinus carpio haematopterus and Cyprinus carpio rubrofuscus) of common carp (Cyprinus carpio L.). A total of 2.4 kb fragment was amplified and subjected to restriction endonuclease analysis with nine restriction endonucleases subsequently. The results indicated that each subspecies owned one hyplotype and four restriction enzymes (Dde I, HaeIII, Taq I and Mbo I) produced diagnostic restriction sites which could be used for discriminating the three subspecies and as molecular genetic markers for assistant selective breeding of common carp.
WOS记录号: WOS:000181790200013
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内容类型: 期刊论文
URI标识: http://ir.ihb.ac.cn/handle/152342/9726
Appears in Collections:中科院水生所知识产出(2009年前)_期刊论文

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Recommended Citation:
Zhou, JF; Wang, ZW; Ye, YZ; Wu, QJ.PCR-RFLP analysis of mitochondrial DNA ND5/6 region among 3 subspecies of common carp (Cyprinus carpio L.) and its application to genetic discrimination of subspecies,CHINESE SCIENCE BULLETIN,2003,48(5):465-468
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