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学科主题: Multidisciplinary Sciences
题名: Identification of antiviral-relevant genes in the cultured fish cells induced by UV-inactivated virus
作者: ZHANG Yibing ; ZHANG Qiya ; XU Dequan ; HU Chengyu ; GUI Jianfang
通讯作者: Gui, JF, Chinese Acad Sci, State Key Lab Freshwater Ecol & Biotechnol, Wuhan Ctr Dev Biol, Inst Hydrobiol, Wuhan 430072, Peoples R China
关键词: antiviral-relevant genes ; immune-relevant genes ; grass carp hemorrhage virus (GCHV) ; interferon ; suppressive subtractive hybridization
刊名: CHINESE SCIENCE BULLETIN
发表日期: 2003
卷: 48, 期:6, 页:581-588
收录类别: SCI
部门归属: Chinese Acad Sci, State Key Lab Freshwater Ecol & Biotechnol, Wuhan Ctr Dev Biol, Inst Hydrobiol, Wuhan 430072, Peoples R China
摘要: UV-inactivated grass carp hemorrhage virus (GCHV) can induce high titer of interferon in cultured CAB (crucian carp (Carassius auratus L.) blastulae) cells, and thus defend host cells against the virus invasion. The mechanism is proposed that an antiviral state should be established in the host cells by activating expression of a set of antiviral-relevant genes. In this study, suppressive subtractive hybridization is applied to constructing a subtracted cDNA library with mRNAs isolated from UV-inactivated GCHV infected and mock-infected CAB cells. 272 differential cDNA fragments are identified by both PCR and dot blot from the subtractive cDNA library. Sequencing analysis reveals 69 genes, including 46 known gene homologues, and 23 unknown putative genes. The known genes include the genes involved in interferon signaling pathways, such as Stat1 and Jak1, the antiviral genes, such as Mx and Viperin, and a set of interferon-stimulated genes observed in mammalian cells. Most of the unknown putative genes contain AU-rich element in their sequences. Differential expressions of these genes are further confirmed by virtual Northern blot and RT-PCR. The data imply that UV-inactivated GCHV is not only able to induce production of interferon in the infected CAB cells, but also leads to the expression of a series of antiviral-relevant genes or immune-relevant genes, and therefore reveals that the signaling pathway of interferon system and antiviral mechanism in fish are similar to those in mammals.
语种: 英语
WOS记录号: WOS:000182036500017
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内容类型: 期刊论文
URI标识: http://ir.ihb.ac.cn/handle/152342/9718
Appears in Collections:中科院水生所知识产出(2009年前)_期刊论文

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ZHANG Yibing; ZHANG Qiya; XU Dequan; HU Chengyu; GUI Jianfang.Identification of antiviral-relevant genes in the cultured fish cells induced by UV-inactivated virus,CHINESE SCIENCE BULLETIN,2003,48(6):581-588
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