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Identification of antiviral-relevant genes in the cultured fish cells induced by UV-inactivated virus
ZHANG Yibing; ZHANG Qiya; XU Dequan; HU Chengyu; GUI Jianfang; Gui, JF, Chinese Acad Sci, State Key Lab Freshwater Ecol & Biotechnol, Wuhan Ctr Dev Biol, Inst Hydrobiol, Wuhan 430072, Peoples R China
2003
Source PublicationCHINESE SCIENCE BULLETIN
Volume48Issue:6Pages:581-588
AbstractUV-inactivated grass carp hemorrhage virus (GCHV) can induce high titer of interferon in cultured CAB (crucian carp (Carassius auratus L.) blastulae) cells, and thus defend host cells against the virus invasion. The mechanism is proposed that an antiviral state should be established in the host cells by activating expression of a set of antiviral-relevant genes. In this study, suppressive subtractive hybridization is applied to constructing a subtracted cDNA library with mRNAs isolated from UV-inactivated GCHV infected and mock-infected CAB cells. 272 differential cDNA fragments are identified by both PCR and dot blot from the subtractive cDNA library. Sequencing analysis reveals 69 genes, including 46 known gene homologues, and 23 unknown putative genes. The known genes include the genes involved in interferon signaling pathways, such as Stat1 and Jak1, the antiviral genes, such as Mx and Viperin, and a set of interferon-stimulated genes observed in mammalian cells. Most of the unknown putative genes contain AU-rich element in their sequences. Differential expressions of these genes are further confirmed by virtual Northern blot and RT-PCR. The data imply that UV-inactivated GCHV is not only able to induce production of interferon in the infected CAB cells, but also leads to the expression of a series of antiviral-relevant genes or immune-relevant genes, and therefore reveals that the signaling pathway of interferon system and antiviral mechanism in fish are similar to those in mammals.
KeywordAntiviral-relevant Genes Immune-relevant Genes Grass Carp Hemorrhage Virus (Gchv) Interferon Suppressive Subtractive Hybridization
DepartmentChinese Acad Sci, State Key Lab Freshwater Ecol & Biotechnol, Wuhan Ctr Dev Biol, Inst Hydrobiol, Wuhan 430072, Peoples R China
Subject AreaMultidisciplinary Sciences
Indexed BySCI
Language英语
WOS IDWOS:000182036500017
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Cited Times:46[WOS]   [WOS Record]     [Related Records in WOS]
Document Type期刊论文
Identifierhttp://ir.ihb.ac.cn/handle/152342/9718
Collection期刊论文
Corresponding AuthorGui, JF, Chinese Acad Sci, State Key Lab Freshwater Ecol & Biotechnol, Wuhan Ctr Dev Biol, Inst Hydrobiol, Wuhan 430072, Peoples R China
Recommended Citation
GB/T 7714
ZHANG Yibing,ZHANG Qiya,XU Dequan,et al. Identification of antiviral-relevant genes in the cultured fish cells induced by UV-inactivated virus[J]. CHINESE SCIENCE BULLETIN,2003,48(6):581-588.
APA ZHANG Yibing,ZHANG Qiya,XU Dequan,HU Chengyu,GUI Jianfang,&Gui, JF, Chinese Acad Sci, State Key Lab Freshwater Ecol & Biotechnol, Wuhan Ctr Dev Biol, Inst Hydrobiol, Wuhan 430072, Peoples R China.(2003).Identification of antiviral-relevant genes in the cultured fish cells induced by UV-inactivated virus.CHINESE SCIENCE BULLETIN,48(6),581-588.
MLA ZHANG Yibing,et al."Identification of antiviral-relevant genes in the cultured fish cells induced by UV-inactivated virus".CHINESE SCIENCE BULLETIN 48.6(2003):581-588.
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