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学科主题: Fisheries; Veterinary Sciences
题名: Detection of Myxobolus rotundus (Myxozoa : Myxosporea) in skin mucus of crucian carp Carassius auratus auratus using a monoclonal antibody
作者: Y. S. Lu ; P. Nie ; B. J. Sun
通讯作者: Nie, P, Chinese Acad Sci, State Key Lab Freshwater Ecol & Biotechnol, Wuhan 430072, Peoples R China
关键词: myxosporean ; Myxobolus rotundus ; crucian carp ; Carassius auratus auratus ; skin mucus ; monoclonal antibody ; diagnosis
刊名: DISEASES OF AQUATIC ORGANISMS
发表日期: 2003
卷: 54, 期:2, 页:171-173
收录类别: SCI
部门归属: Chinese Acad Sci, State Key Lab Freshwater Ecol & Biotechnol, Wuhan 430072, Peoples R China; Chinese Acad Sci, Lab Fish Dis, Inst Hydrol, Wuhan 430072, Peoples R China
摘要: Diagnosis of myxosporean Myxobolus rotundus infection was conducted by examining skin mucus from the infected crucian carp Carassius auratus auratus with a monoclonal antibody, MAb 2D12, raised previously against the parasite. A positive reaction was observed in skin mucus collected from infected fish, and spores and pre-spore stages of the parasite were identified by the MAb 2D12. It was also demonstrated that M. rotundus infection can be successfully detected by a simple method, enzyme-linked immunosorbent assay (ELISA), and that skin mucus collected from infected fish skin had a significantly higher optical density (OD) value than that from uninfected fish.
WOS记录号: WOS:000183491500010
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内容类型: 期刊论文
URI标识: http://ir.ihb.ac.cn/handle/152342/9690
Appears in Collections:中科院水生所知识产出(2009年前)_期刊论文

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Recommended Citation:
Y. S. Lu; P. Nie; B. J. Sun.Detection of Myxobolus rotundus (Myxozoa : Myxosporea) in skin mucus of crucian carp Carassius auratus auratus using a monoclonal antibody,DISEASES OF AQUATIC ORGANISMS,2003,54(2):171-173
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文件名: Detection of Myxobolus rotundus (Myxozoa-Myxosporea) in skin mucus of crucian carp Carassius auratus auratus using a monoclonal antibody.pdf
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