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Detection of Myxobolus rotundus (Myxozoa : Myxosporea) in skin mucus of crucian carp Carassius auratus auratus using a monoclonal antibody
Y. S. Lu; P. Nie; B. J. Sun; Nie, P, Chinese Acad Sci, State Key Lab Freshwater Ecol & Biotechnol, Wuhan 430072, Peoples R China
2003
Source PublicationDISEASES OF AQUATIC ORGANISMS
Volume54Issue:2Pages:171-173
AbstractDiagnosis of myxosporean Myxobolus rotundus infection was conducted by examining skin mucus from the infected crucian carp Carassius auratus auratus with a monoclonal antibody, MAb 2D12, raised previously against the parasite. A positive reaction was observed in skin mucus collected from infected fish, and spores and pre-spore stages of the parasite were identified by the MAb 2D12. It was also demonstrated that M. rotundus infection can be successfully detected by a simple method, enzyme-linked immunosorbent assay (ELISA), and that skin mucus collected from infected fish skin had a significantly higher optical density (OD) value than that from uninfected fish.
KeywordMyxosporean Myxobolus Rotundus Crucian Carp Carassius Auratus Auratus Skin Mucus Monoclonal Antibody Diagnosis
DepartmentChinese Acad Sci, State Key Lab Freshwater Ecol & Biotechnol, Wuhan 430072, Peoples R China; Chinese Acad Sci, Lab Fish Dis, Inst Hydrol, Wuhan 430072, Peoples R China
Subject AreaFisheries ; Veterinary Sciences
Indexed BySCI
WOS IDWOS:000183491500010
Citation statistics
Cited Times:4[WOS]   [WOS Record]     [Related Records in WOS]
Document Type期刊论文
Identifierhttp://ir.ihb.ac.cn/handle/152342/9690
Collection期刊论文
Corresponding AuthorNie, P, Chinese Acad Sci, State Key Lab Freshwater Ecol & Biotechnol, Wuhan 430072, Peoples R China
Recommended Citation
GB/T 7714
Y. S. Lu,P. Nie,B. J. Sun,et al. Detection of Myxobolus rotundus (Myxozoa : Myxosporea) in skin mucus of crucian carp Carassius auratus auratus using a monoclonal antibody[J]. DISEASES OF AQUATIC ORGANISMS,2003,54(2):171-173.
APA Y. S. Lu,P. Nie,B. J. Sun,&Nie, P, Chinese Acad Sci, State Key Lab Freshwater Ecol & Biotechnol, Wuhan 430072, Peoples R China.(2003).Detection of Myxobolus rotundus (Myxozoa : Myxosporea) in skin mucus of crucian carp Carassius auratus auratus using a monoclonal antibody.DISEASES OF AQUATIC ORGANISMS,54(2),171-173.
MLA Y. S. Lu,et al."Detection of Myxobolus rotundus (Myxozoa : Myxosporea) in skin mucus of crucian carp Carassius auratus auratus using a monoclonal antibody".DISEASES OF AQUATIC ORGANISMS 54.2(2003):171-173.
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