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Agrobacterium-mediated transformation of Kentucky bluegrass
Chai, BF; Liang, AH; Wang, W; Wei, H; Chai, BF, Chinese Acad Sci, Inst Hydrobiol, Wuhan 430072, Peoples R China
AbstractEmbryogenic calli of Kentucky bluegrass, named Md, were induced from mature seeds and embryos, and proliferated on medium K3 containing 2,4-dichlorophenoxyacetic acid (2,4-D, 10.0 mumol/L), 6-benzylaminopurine (BAR, 0.5 mumol/L) and K5 which was the K3 medium supplemented with cupric sulfa (0.5 mumol/L) under dim-light condition (20-30 mumol.m(-2).s-1, 16 h light) at 24 degreesC. Embryogenic calli were transformed with plasmids pDM805 Carring bar and gus genes, Which was mediated by an Agrobacterium strain AGL1, four transgenic lines were obtained. The important factors that affect the transformation efficiency and obtain desirable number of transgenic plants included: (1) the quality of embryogenic calli; (2) light condition and time of co-cultivation; (3) concentration of antibiotics used for suppressing the overgrowth of Agrobacterium in the course of transformed plant regeneration; (4) selection pressure, etc. The micro nutrient of cupric had significant influence on the quality of embryogenic calli. This presentation is the first successful protocol of Kentucky bluegrass transformation mediated by Agrobacterium.
KeywordMonocotyledon Callus Agrobacterium-mediation Poa Pratensis
DepartmentChinese Acad Sci, Inst Hydrobiol, Wuhan 430072, Peoples R China; Shanxi Agr Univ, Inst Biotechnol, Taiyuan 030006, Peoples R China
Subject AreaBiochemistry & Molecular Biology ; Plant Sciences
Indexed BySCI
WOS IDWOS:000184913500013
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Cited Times:7[WOS]   [WOS Record]     [Related Records in WOS]
Document Type期刊论文
Corresponding AuthorChai, BF, Chinese Acad Sci, Inst Hydrobiol, Wuhan 430072, Peoples R China
Recommended Citation
GB/T 7714
Chai, BF,Liang, AH,Wang, W,et al. Agrobacterium-mediated transformation of Kentucky bluegrass[J]. ACTA BOTANICA SINICA,2003,45(8):966-973.
APA Chai, BF,Liang, AH,Wang, W,Wei, H,&Chai, BF, Chinese Acad Sci, Inst Hydrobiol, Wuhan 430072, Peoples R China.(2003).Agrobacterium-mediated transformation of Kentucky bluegrass.ACTA BOTANICA SINICA,45(8),966-973.
MLA Chai, BF,et al."Agrobacterium-mediated transformation of Kentucky bluegrass".ACTA BOTANICA SINICA 45.8(2003):966-973.
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