| Agrobacterium-mediated transformation of Kentucky bluegrass | |
| Chai, BF; Liang, AH; Wang, W; Wei, H; Chai, BF, Chinese Acad Sci, Inst Hydrobiol, Wuhan 430072, Peoples R China | |
| 2003 | |
| Source Publication | ACTA BOTANICA SINICA
 |
| Volume | 45Issue:8Pages:966-973 |
| Abstract | Embryogenic calli of Kentucky bluegrass, named Md, were induced from mature seeds and embryos, and proliferated on medium K3 containing 2,4-dichlorophenoxyacetic acid (2,4-D, 10.0 mumol/L), 6-benzylaminopurine (BAR, 0.5 mumol/L) and K5 which was the K3 medium supplemented with cupric sulfa (0.5 mumol/L) under dim-light condition (20-30 mumol.m(-2).s-1, 16 h light) at 24 degreesC. Embryogenic calli were transformed with plasmids pDM805 Carring bar and gus genes, Which was mediated by an Agrobacterium strain AGL1, four transgenic lines were obtained. The important factors that affect the transformation efficiency and obtain desirable number of transgenic plants included: (1) the quality of embryogenic calli; (2) light condition and time of co-cultivation; (3) concentration of antibiotics used for suppressing the overgrowth of Agrobacterium in the course of transformed plant regeneration; (4) selection pressure, etc. The micro nutrient of cupric had significant influence on the quality of embryogenic calli. This presentation is the first successful protocol of Kentucky bluegrass transformation mediated by Agrobacterium. |
| Keyword | Monocotyledon Callus Agrobacterium-mediation Poa Pratensis |
| Department | Chinese Acad Sci, Inst Hydrobiol, Wuhan 430072, Peoples R China; Shanxi Agr Univ, Inst Biotechnol, Taiyuan 030006, Peoples R China |
| Subject Area | Biochemistry & Molecular Biology ; Plant Sciences |
| Indexed By | SCI |
| WOS ID | WOS:000184913500013 |
| Citation statistics | |
| Document Type | 期刊论文 |
| Identifier | http://ir.ihb.ac.cn/handle/152342/9666 |
| Collection | 期刊论文 |
| Corresponding Author | Chai, BF, Chinese Acad Sci, Inst Hydrobiol, Wuhan 430072, Peoples R China |
| Recommended Citation GB/T 7714 | Chai, BF,Liang, AH,Wang, W,et al. Agrobacterium-mediated transformation of Kentucky bluegrass[J]. ACTA BOTANICA SINICA,2003,45(8):966-973. |
| APA | Chai, BF,Liang, AH,Wang, W,Wei, H,&Chai, BF, Chinese Acad Sci, Inst Hydrobiol, Wuhan 430072, Peoples R China.(2003).Agrobacterium-mediated transformation of Kentucky bluegrass.ACTA BOTANICA SINICA,45(8),966-973. |
| MLA | Chai, BF,et al."Agrobacterium-mediated transformation of Kentucky bluegrass".ACTA BOTANICA SINICA 45.8(2003):966-973. |
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