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学科主题: Biochemical Research Methods; Immunology
题名: Construction and characterization of a novel recombinant single-chain variable fragment antibody against White Spot Syndrome Virus from shrimp
作者: Dai, HP; Gao, H; Zhao, XY; Dai, LF; Zhang, XK; Xiao, N; Zhao, RH; Hemmingsen, SM
通讯作者: Dai, HP, Chinese Acad Sci, Inst Hydrobiol, E Lake So Rd 7 Wuchan, Wuhan 430072, Hubei, Peoples R China
关键词: phage display ; White Spot Syndrome Virus ; shrimp
刊名: JOURNAL OF IMMUNOLOGICAL METHODS
发表日期: 2003-08-01
DOI: 10.1016/S0022-1759(03)00182-0
卷: 279, 期:1-2, 页:267-275
收录类别: SCI
文章类型: Article
部门归属: Chinese Acad Sci, Inst Hydrobiol, Wuhan 430072, Hubei, Peoples R China; Natl Res Council Canada, Inst Plant Biotechnol, Saskatoon, SK S7N 0W9, Canada
WOS标题词: Science & Technology ; Life Sciences & Biomedicine
类目[WOS]: Biochemical Research Methods ; Immunology
研究领域[WOS]: Biochemistry & Molecular Biology ; Immunology
摘要: An antibody phage display library against White Spot Syndrome Virus (WSSV) was constructed. After four rounds of panning against WSSV, 192 out of 480 clones displayed WSSV binding activity. One of the positive clones, designated A1, had relatively higher activity specifically binding to WSSV A1-soluble, single-chain fragment variable (scFv) antibody has an affinity constant (K-aff) of 2.02 +/- 0.42 x 10(9) M-1. Dot blot assays showed that A1-soluble scFv could detect WSSV directly from shrimp hemolymph after 24-h feeding infection by WSSV. A1 scFv has potential for the development of a cheap, simple and sensitive diagnostic kit for WSSV in the field. (C) 2003 Elsevier Science B.V. All rights reserved.
英文摘要: An antibody phage display library against White Spot Syndrome Virus (WSSV) was constructed. After four rounds of panning against WSSV, 192 out of 480 clones displayed WSSV binding activity. One of the positive clones, designated A1, had relatively higher activity specifically binding to WSSV A1-soluble, single-chain fragment variable (scFv) antibody has an affinity constant (K-aff) of 2.02 +/- 0.42 x 10(9) M-1. Dot blot assays showed that A1-soluble scFv could detect WSSV directly from shrimp hemolymph after 24-h feeding infection by WSSV. A1 scFv has potential for the development of a cheap, simple and sensitive diagnostic kit for WSSV in the field. (C) 2003 Elsevier Science B.V. All rights reserved.
关键词[WOS]: PHAGE DISPLAY ; GENOME SEQUENCE ; PENAEID SHRIMP ; BACULOVIRUS ; POLYMERASE
语种: 英语
WOS记录号: WOS:000185381300024
ISSN号: 0022-1759
Citation statistics:
内容类型: 期刊论文
URI标识: http://ir.ihb.ac.cn/handle/152342/9650
Appears in Collections:中科院水生所知识产出(2009年前)_期刊论文

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作者单位: 1.Chinese Acad Sci, Inst Hydrobiol, Wuhan 430072, Hubei, Peoples R China
2.Natl Res Council Canada, Inst Plant Biotechnol, Saskatoon, SK S7N 0W9, Canada

Recommended Citation:
Dai, HP; Gao, H; Zhao, XY; Dai, LF; Zhang, XK; Xiao, N; Zhao, RH; Hemmingsen, SM.Construction and characterization of a novel recombinant single-chain variable fragment antibody against White Spot Syndrome Virus from shrimp,JOURNAL OF IMMUNOLOGICAL METHODS,2003,279(1-2):267-275
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