IHB OpenIR  > 期刊论文
Construction and characterization of a novel recombinant single-chain variable fragment antibody against White Spot Syndrome Virus from shrimp
Dai, HP; Gao, H; Zhao, XY; Dai, LF; Zhang, XK; Xiao, N; Zhao, RH; Hemmingsen, SM; Dai, HP, Chinese Acad Sci, Inst Hydrobiol, E Lake So Rd 7 Wuchan, Wuhan 430072, Hubei, Peoples R China
2003
Source PublicationJOURNAL OF IMMUNOLOGICAL METHODS
Volume279Issue:1-2Pages:267-275
AbstractAn antibody phage display library against White Spot Syndrome Virus (WSSV) was constructed. After four rounds of panning against WSSV, 192 out of 480 clones displayed WSSV binding activity. One of the positive clones, designated A1, had relatively higher activity specifically binding to WSSV A1-soluble, single-chain fragment variable (scFv) antibody has an affinity constant (K-aff) of 2.02 +/- 0.42 x 10(9) M-1. Dot blot assays showed that A1-soluble scFv could detect WSSV directly from shrimp hemolymph after 24-h feeding infection by WSSV. A1 scFv has potential for the development of a cheap, simple and sensitive diagnostic kit for WSSV in the field. (C) 2003 Elsevier Science B.V. All rights reserved.
KeywordPhage Display White Spot Syndrome Virus Shrimp
DepartmentChinese Acad Sci, Inst Hydrobiol, Wuhan 430072, Hubei, Peoples R China; Natl Res Council Canada, Inst Plant Biotechnol, Saskatoon, SK S7N 0W9, Canada
Subject AreaBiochemical Research Methods ; Immunology
Indexed BySCI
WOS IDWOS:000185381300024
Citation statistics
Document Type期刊论文
Identifierhttp://ir.ihb.ac.cn/handle/152342/9650
Collection期刊论文
Corresponding AuthorDai, HP, Chinese Acad Sci, Inst Hydrobiol, E Lake So Rd 7 Wuchan, Wuhan 430072, Hubei, Peoples R China
Recommended Citation
GB/T 7714
Dai, HP,Gao, H,Zhao, XY,et al. Construction and characterization of a novel recombinant single-chain variable fragment antibody against White Spot Syndrome Virus from shrimp[J]. JOURNAL OF IMMUNOLOGICAL METHODS,2003,279(1-2):267-275.
APA Dai, HP.,Gao, H.,Zhao, XY.,Dai, LF.,Zhang, XK.,...&Dai, HP, Chinese Acad Sci, Inst Hydrobiol, E Lake So Rd 7 Wuchan, Wuhan 430072, Hubei, Peoples R China.(2003).Construction and characterization of a novel recombinant single-chain variable fragment antibody against White Spot Syndrome Virus from shrimp.JOURNAL OF IMMUNOLOGICAL METHODS,279(1-2),267-275.
MLA Dai, HP,et al."Construction and characterization of a novel recombinant single-chain variable fragment antibody against White Spot Syndrome Virus from shrimp".JOURNAL OF IMMUNOLOGICAL METHODS 279.1-2(2003):267-275.
Files in This Item:
File Name/Size DocType Version Access License
Construction and cha(519KB) 开放获取--View Application Full Text
Related Services
Recommend this item
Bookmark
Usage statistics
Export to Endnote
Google Scholar
Similar articles in Google Scholar
[Dai, HP]'s Articles
[Gao, H]'s Articles
[Zhao, XY]'s Articles
Baidu academic
Similar articles in Baidu academic
[Dai, HP]'s Articles
[Gao, H]'s Articles
[Zhao, XY]'s Articles
Bing Scholar
Similar articles in Bing Scholar
[Dai, HP]'s Articles
[Gao, H]'s Articles
[Zhao, XY]'s Articles
Terms of Use
No data!
Social Bookmark/Share
File name: Construction and characterization of a novel recombinant single-chain variable fragment antibody against White Spot Syndrome Virus from shrimp.pdf
Format: Adobe PDF
This file does not support browsing at this time
All comments (0)
No comment.
 

Items in the repository are protected by copyright, with all rights reserved, unless otherwise indicated.