IHB OpenIR  > 期刊论文
Characterization of cDNA encoding immunoglobulin light chain of the Mandarin fish (Siniperca chuatsi)
Zhang, YA; Nie, P; Luo, HY; Wang, YP; Sun, YH; Zhu, ZY; Nie, P, Chinese Acad Sci, State Key Lab Freshwater Ecol & Biotechnol, Wuhan 430072, Hubei Province, Peoples R China
2003
Source PublicationVETERINARY IMMUNOLOGY AND IMMUNOPATHOLOGY
Volume95Issue:1-2Pages:81-90
AbstractImmunoglobulin light chain cDNA sequences of a perciform fish, the mandarin fish Siniperca chuatsi were amplified from head kidney mRNA by reverse transcription (RT)-PCR and RACE methods using degenerated primer and gene specific ones. In cDNA sequences of the VL region, nucleotide exchanges were present mainly within CDRs, although a lesser degree of variability was also found in FRs. Moreover, the length of CDRI and CDR3 in the mandarin fish is shorter than in most other fish species. In the middle of S. chuatsi CL region, a microsatellite sequence (AGC)(6-8) was found, which is also present in another perciform species, the spotted wolffish (Anarhichas minor). The comparison of amino acid sequence of the mandarin fish CL domain with those of other vertebrates showed the highest degree of similarity of 94.5% to the spotted wolffish, while the similarity with rainbow trout (Oncorhynchus mykiss) Ig L1 (62.7%) and channel catfish (Ictalurus punctatus) Ig LG (55.9%) isotypes is also higher. However, there is only 50% identity in the VL regions between the mandarin fish and the wolffish. The sequence similarity of the mandarin fish CL domain with those of higher vertebrate did not readily allow it to be classified as kappa or lambda isotype. The phylogenetic analyses also demonstrated that the CL genes of the mandarin fish and most other teleost fish cluster as a separate branch out of the mammal kappa and lambda branches. (C) 2003 Elsevier B.V. All rights reserved.
KeywordImmunoglobulin Cdna Sequence Light Chain Mandarin Fish Chinese Perch Simperca Chuatsi
DepartmentChinese Acad Sci, State Key Lab Freshwater Ecol & Biotechnol, Wuhan 430072, Hubei Province, Peoples R China; Chinese Acad Sci, Lab Fish Dis, Inst Hydrobiol, Wuhan 430072, Hubei Province, Peoples R China
Subject AreaImmunology ; Veterinary Sciences
Indexed BySCI
WOS IDWOS:000185530500009
Citation statistics
Cited Times:19[WOS]   [WOS Record]     [Related Records in WOS]
Document Type期刊论文
Identifierhttp://ir.ihb.ac.cn/handle/152342/9644
Collection期刊论文
Corresponding AuthorNie, P, Chinese Acad Sci, State Key Lab Freshwater Ecol & Biotechnol, Wuhan 430072, Hubei Province, Peoples R China
Recommended Citation
GB/T 7714
Zhang, YA,Nie, P,Luo, HY,et al. Characterization of cDNA encoding immunoglobulin light chain of the Mandarin fish (Siniperca chuatsi)[J]. VETERINARY IMMUNOLOGY AND IMMUNOPATHOLOGY,2003,95(1-2):81-90.
APA Zhang, YA.,Nie, P.,Luo, HY.,Wang, YP.,Sun, YH.,...&Nie, P, Chinese Acad Sci, State Key Lab Freshwater Ecol & Biotechnol, Wuhan 430072, Hubei Province, Peoples R China.(2003).Characterization of cDNA encoding immunoglobulin light chain of the Mandarin fish (Siniperca chuatsi).VETERINARY IMMUNOLOGY AND IMMUNOPATHOLOGY,95(1-2),81-90.
MLA Zhang, YA,et al."Characterization of cDNA encoding immunoglobulin light chain of the Mandarin fish (Siniperca chuatsi)".VETERINARY IMMUNOLOGY AND IMMUNOPATHOLOGY 95.1-2(2003):81-90.
Files in This Item:
File Name/Size DocType Version Access License
Characterization of (227KB) 开放获取--View Application Full Text
Related Services
Recommend this item
Bookmark
Usage statistics
Export to Endnote
Google Scholar
Similar articles in Google Scholar
[Zhang, YA]'s Articles
[Nie, P]'s Articles
[Luo, HY]'s Articles
Baidu academic
Similar articles in Baidu academic
[Zhang, YA]'s Articles
[Nie, P]'s Articles
[Luo, HY]'s Articles
Bing Scholar
Similar articles in Bing Scholar
[Zhang, YA]'s Articles
[Nie, P]'s Articles
[Luo, HY]'s Articles
Terms of Use
No data!
Social Bookmark/Share
File name: Characterization of cDNA encoding immunoglobulin light chain of the Mandarin fish (Siniperca chuatsi).pdf
Format: Adobe PDF
This file does not support browsing at this time
All comments (0)
No comment.
 

Items in the repository are protected by copyright, with all rights reserved, unless otherwise indicated.