中国科学院水生生物研究所机构知识库
Advanced  
IHB OpenIR  > 中科院水生所知识产出(2009年前)  > 期刊论文
学科主题: Immunology; Veterinary Sciences
题名: Characterization of cDNA encoding immunoglobulin light chain of the Mandarin fish (Siniperca chuatsi)
作者: Zhang, YA; Nie, P; Luo, HY; Wang, YP; Sun, YH; Zhu, ZY
通讯作者: Nie, P, Chinese Acad Sci, State Key Lab Freshwater Ecol & Biotechnol, Wuhan 430072, Hubei Province, Peoples R China
关键词: immunoglobulin ; cDNA sequence ; light chain ; Mandarin fish ; Chinese perch ; Simperca chuatsi
刊名: VETERINARY IMMUNOLOGY AND IMMUNOPATHOLOGY
发表日期: 2003-09-15
DOI: 10.1016/S0165-2427(03)00105-3
卷: 95, 期:1-2, 页:81-90
收录类别: SCI
文章类型: Article
部门归属: Chinese Acad Sci, State Key Lab Freshwater Ecol & Biotechnol, Wuhan 430072, Hubei Province, Peoples R China; Chinese Acad Sci, Lab Fish Dis, Inst Hydrobiol, Wuhan 430072, Hubei Province, Peoples R China
WOS标题词: Science & Technology ; Life Sciences & Biomedicine
类目[WOS]: Immunology ; Veterinary Sciences
研究领域[WOS]: Immunology ; Veterinary Sciences
摘要: Immunoglobulin light chain cDNA sequences of a perciform fish, the mandarin fish Siniperca chuatsi were amplified from head kidney mRNA by reverse transcription (RT)-PCR and RACE methods using degenerated primer and gene specific ones. In cDNA sequences of the VL region, nucleotide exchanges were present mainly within CDRs, although a lesser degree of variability was also found in FRs. Moreover, the length of CDRI and CDR3 in the mandarin fish is shorter than in most other fish species. In the middle of S. chuatsi CL region, a microsatellite sequence (AGC)(6-8) was found, which is also present in another perciform species, the spotted wolffish (Anarhichas minor). The comparison of amino acid sequence of the mandarin fish CL domain with those of other vertebrates showed the highest degree of similarity of 94.5% to the spotted wolffish, while the similarity with rainbow trout (Oncorhynchus mykiss) Ig L1 (62.7%) and channel catfish (Ictalurus punctatus) Ig LG (55.9%) isotypes is also higher. However, there is only 50% identity in the VL regions between the mandarin fish and the wolffish. The sequence similarity of the mandarin fish CL domain with those of higher vertebrate did not readily allow it to be classified as kappa or lambda isotype. The phylogenetic analyses also demonstrated that the CL genes of the mandarin fish and most other teleost fish cluster as a separate branch out of the mammal kappa and lambda branches. (C) 2003 Elsevier B.V. All rights reserved.
英文摘要: Immunoglobulin light chain cDNA sequences of a perciform fish, the mandarin fish Siniperca chuatsi were amplified from head kidney mRNA by reverse transcription (RT)-PCR and RACE methods using degenerated primer and gene specific ones. In cDNA sequences of the VL region, nucleotide exchanges were present mainly within CDRs, although a lesser degree of variability was also found in FRs. Moreover, the length of CDRI and CDR3 in the mandarin fish is shorter than in most other fish species. In the middle of S. chuatsi CL region, a microsatellite sequence (AGC)(6-8) was found, which is also present in another perciform species, the spotted wolffish (Anarhichas minor). The comparison of amino acid sequence of the mandarin fish CL domain with those of other vertebrates showed the highest degree of similarity of 94.5% to the spotted wolffish, while the similarity with rainbow trout (Oncorhynchus mykiss) Ig L1 (62.7%) and channel catfish (Ictalurus punctatus) Ig LG (55.9%) isotypes is also higher. However, there is only 50% identity in the VL regions between the mandarin fish and the wolffish. The sequence similarity of the mandarin fish CL domain with those of higher vertebrate did not readily allow it to be classified as kappa or lambda isotype. The phylogenetic analyses also demonstrated that the CL genes of the mandarin fish and most other teleost fish cluster as a separate branch out of the mammal kappa and lambda branches. (C) 2003 Elsevier B.V. All rights reserved.
关键词[WOS]: CHANNEL CATFISH ; RAINBOW-TROUT ; HEAVY-CHAIN ; GENOMIC ORGANIZATION ; SIBERIAN STURGEON ; NUCLEAR PROTEINS ; ACIPENSER BAERI ; GENES ; L. ; VARIABILITY
语种: 英语
WOS记录号: WOS:000185530500009
ISSN号: 0165-2427
Citation statistics:
内容类型: 期刊论文
URI标识: http://ir.ihb.ac.cn/handle/152342/9644
Appears in Collections:中科院水生所知识产出(2009年前)_期刊论文

Files in This Item: Download All
File Name/ File Size Content Type Version Access License
Characterization of cDNA encoding immunoglobulin light chain of the Mandarin fish (Siniperca chuatsi).pdf(227KB)----开放获取--View Download

作者单位: 1.Chinese Acad Sci, State Key Lab Freshwater Ecol & Biotechnol, Wuhan 430072, Hubei Province, Peoples R China
2.Chinese Acad Sci, Lab Fish Dis, Inst Hydrobiol, Wuhan 430072, Hubei Province, Peoples R China

Recommended Citation:
Zhang, YA; Nie, P; Luo, HY; Wang, YP; Sun, YH; Zhu, ZY.Characterization of cDNA encoding immunoglobulin light chain of the Mandarin fish (Siniperca chuatsi),VETERINARY IMMUNOLOGY AND IMMUNOPATHOLOGY,2003,95(1-2):81-90
Service
Recommend this item
Sava as my favorate item
Show this item's statistics
Export Endnote File
Google Scholar
Similar articles in Google Scholar
[Zhang, YA]'s Articles
[Nie, P]'s Articles
[Luo, HY]'s Articles
CSDL cross search
Similar articles in CSDL Cross Search
[Zhang, YA]‘s Articles
[Nie, P]‘s Articles
[Luo, HY]‘s Articles
Related Copyright Policies
Null
Social Bookmarking
Add to CiteULike Add to Connotea Add to Del.icio.us Add to Digg Add to Reddit
文件名: Characterization of cDNA encoding immunoglobulin light chain of the Mandarin fish (Siniperca chuatsi).pdf
格式: Adobe PDF
此文件暂不支持浏览
所有评论 (0)
暂无评论
 
评注功能仅针对注册用户开放,请您登录
您对该条目有什么异议,请填写以下表单,管理员会尽快联系您。
内 容:
Email:  *
单位:
验证码:   刷新
您在IR的使用过程中有什么好的想法或者建议可以反馈给我们。
标 题:
 *
内 容:
Email:  *
验证码:   刷新

Items in IR are protected by copyright, with all rights reserved, unless otherwise indicated.

 

 

Valid XHTML 1.0!
Copyright © 2007-2017  中国科学院水生生物研究所 - Feedback
Powered by CSpace