| Molecular cytogenetic detection of paternal chromosome fragments in allogynogenetic gibel carp, Carassius auratus gibelio Bloch | |
| M. S. Yi; Y. Q. Li; J. D. Liu; L. Zhou; Q. X. Yu & J. F. Gui; Gui, JF, Chinese Acad Sci, Inst Hydrobiol, State Key Lab Freshwater Ecol & Biotechnol, Wuhan 430072, Peoples R China | |
| 2003 | |
| Source Publication | CHROMOSOME RESEARCH
 |
| Volume | 11Issue:7Pages:665-671 |
| Abstract | In gynogenesis, sperm from related species activates egg and embryonic development, but normally does not contribute genetically to the offspring. In gibel carp, Carassius auratus gibelio Bloch, however, gynogenetic offspring often show some phenotypes apparently derived from the heterologous sperm donor. This paternal effect of allogynogenesis is outstanding in an artificial clone F produced by cold treatment of clone E eggs after insemination with blunt-nose black bream (Megaloabrama amblycephala Yin) sperm. Karyotype analysis revealed 5-15 supernumerary microchromosomes in different individuals of clone F in addition to 156 normal chromosomes inherited from the maternal clone E. A painting probe was prepared from the microdissected microchromosomes, and used to investigate the origin of these microchromosomes. Strong positive signals were detected on each microchromosomes of clone F and on 4 pairs of chromosomes in blunt-nose black bream, whereas no signals were detected on the chromosomes of clone E. This result indicates that some paternal chromosome fragments of blunt-nose black bream have been incorporated into the artificial clone F. Therefore, the manipulation of allogynogenesis may provide a unique method to transfer DNA between diverse species for fish breeding. |
| Keyword | Allogynogenesis Chromosome Painting Microchromosome Microdissection Paternal Chromosome Fragment |
| Department | Chinese Acad Sci, Inst Hydrobiol, State Key Lab Freshwater Ecol & Biotechnol, Wuhan 430072, Peoples R China; Hubei Univ, Fac Life Sci, Wuhan 430062, Peoples R China; Wuhan Univ, Sch Life Sci, Wuhan 430072, Peoples R China |
| Subject Area | Biochemistry & Molecular Biology ; Genetics & Heredity |
| Indexed By | SCI |
| WOS ID | WOS:000185621500003 |
| Citation statistics | |
| Document Type | 期刊论文 |
| Identifier | http://ir.ihb.ac.cn/handle/152342/9642 |
| Collection | 期刊论文 |
| Corresponding Author | Gui, JF, Chinese Acad Sci, Inst Hydrobiol, State Key Lab Freshwater Ecol & Biotechnol, Wuhan 430072, Peoples R China |
| Recommended Citation GB/T 7714 | M. S. Yi,Y. Q. Li,J. D. Liu,et al. Molecular cytogenetic detection of paternal chromosome fragments in allogynogenetic gibel carp, Carassius auratus gibelio Bloch[J]. CHROMOSOME RESEARCH,2003,11(7):665-671. |
| APA | M. S. Yi,Y. Q. Li,J. D. Liu,L. Zhou,Q. X. Yu & J. F. Gui,&Gui, JF, Chinese Acad Sci, Inst Hydrobiol, State Key Lab Freshwater Ecol & Biotechnol, Wuhan 430072, Peoples R China.(2003).Molecular cytogenetic detection of paternal chromosome fragments in allogynogenetic gibel carp, Carassius auratus gibelio Bloch.CHROMOSOME RESEARCH,11(7),665-671. |
| MLA | M. S. Yi,et al."Molecular cytogenetic detection of paternal chromosome fragments in allogynogenetic gibel carp, Carassius auratus gibelio Bloch".CHROMOSOME RESEARCH 11.7(2003):665-671. |
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