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Molecular cloning and characterization of crucian carp (Carassius auratus L.) interferon regulatory factor 7
Yi-bing Zhang; Cheng-yu Hu; Jing Zhang; Guo-ping Huang; Li-hua Wei; Qi-ya Zhang; Jian-fang Gui; Gui, JF, Chinese Acad Sci, Inst Hydrobiol, Dept Fish Genet & Breeding, Wuhan 430072, Hubei Province, Peoples R China
2003
Source PublicationFISH & SHELLFISH IMMUNOLOGY
Volume15Issue:5Pages:453-466
AbstractInterferon (IFN) can induce an antiviral state via interferon-regulatory transcription factors (IRFs), which bind to and control genes directed by the interferon-stimulated response element (ISRE). Here we describe a fish IRF, termed CaIRF7, cloned from a subtractive cDNA library which is constructed with mRNAs obtained from crucian carp (Carassius auratus L.) blastulae embryonic (CAB) cells infected by UV-inactivated GCHV and mock-infected cells. CaIRF7 cDNA was found to be 1816 bp in length, with a 42 bp 5' UTR and a 508 bp 3' UTR. The open reading frame translates into 421 amino acids in which a DNA-binding domain (DBD) containing the repeated tryptophan motif and IRFs association domain have been identified. Like chicken GgIRF3, CaIRF7 was most similar to mammalian IRF7 with 27 to 30% identity overall and some 37% identity in their DBDs. A single transcript of 1.9 kb was detected in virally induced CAB cells by virtual Northern blotting. RT-PCR analysis revealed a wide tissue distribution of CaIRF7 constitutive expression, with detectable transcript in non-infected CAB cells and various tissues of healthy crucian carp. In addition, CaIRF7 expression was differentially increased by stimulation of the CAB cells with active GCHV, UV-inactivated GCHV or CAB IFN, indicating that the activation of CaIRF7 was directly regulated by IFN. (C) 2003 Published by Elsevier Ltd.
KeywordInterferon Regulatory Factor (Irf) Cdna Crucian Carp Blastulae Embryonic (Cab) Cells Gchv Immune-related Genes Antiviral-relevant Genes
DepartmentChinese Acad Sci, Inst Hydrobiol, State Key Lab Freshwater Ecol & Biotechnol, Wuhan Ctr Dev Biol, Wuhan 430072, Hubei Province, Peoples R China
Subject AreaFisheries ; Immunology ; Marine & Freshwater Biology ; Veterinary Sciences
Indexed BySCI
WOS IDWOS:000186734400008
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Cited Times:70[WOS]   [WOS Record]     [Related Records in WOS]
Document Type期刊论文
Identifierhttp://ir.ihb.ac.cn/handle/152342/9610
Collection期刊论文
Corresponding AuthorGui, JF, Chinese Acad Sci, Inst Hydrobiol, Dept Fish Genet & Breeding, Wuhan 430072, Hubei Province, Peoples R China
Recommended Citation
GB/T 7714
Yi-bing Zhang,Cheng-yu Hu,Jing Zhang,et al. Molecular cloning and characterization of crucian carp (Carassius auratus L.) interferon regulatory factor 7[J]. FISH & SHELLFISH IMMUNOLOGY,2003,15(5):453-466.
APA Yi-bing Zhang.,Cheng-yu Hu.,Jing Zhang.,Guo-ping Huang.,Li-hua Wei.,...&Gui, JF, Chinese Acad Sci, Inst Hydrobiol, Dept Fish Genet & Breeding, Wuhan 430072, Hubei Province, Peoples R China.(2003).Molecular cloning and characterization of crucian carp (Carassius auratus L.) interferon regulatory factor 7.FISH & SHELLFISH IMMUNOLOGY,15(5),453-466.
MLA Yi-bing Zhang,et al."Molecular cloning and characterization of crucian carp (Carassius auratus L.) interferon regulatory factor 7".FISH & SHELLFISH IMMUNOLOGY 15.5(2003):453-466.
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