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学科主题: Fisheries; Immunology; Marine & Freshwater Biology; Veterinary Sciences
题名: Molecular cloning and characterization of crucian carp (Carassius auratus L.) interferon regulatory factor 7
作者: Zhang, YB; Hu, CY; Zhang, J; Huang, GP; Wei, LH; Zhang, QY; Gui, JF
通讯作者: Gui, JF, Chinese Acad Sci, Inst Hydrobiol, Dept Fish Genet & Breeding, Wuhan 430072, Hubei Province, Peoples R China
关键词: interferon regulatory factor (IRF) ; cDNA ; crucian carp blastulae embryonic (CAB) cells ; GCHV ; immune-related genes ; antiviral-relevant genes
刊名: FISH & SHELLFISH IMMUNOLOGY
发表日期: 2003-11-01
DOI: 10.1016/S1050-4648(03)00025-1
卷: 15, 期:5, 页:453-466
收录类别: SCI
文章类型: Article
部门归属: Chinese Acad Sci, Inst Hydrobiol, State Key Lab Freshwater Ecol & Biotechnol, Wuhan Ctr Dev Biol, Wuhan 430072, Hubei Province, Peoples R China
WOS标题词: Science & Technology ; Life Sciences & Biomedicine
类目[WOS]: Fisheries ; Immunology ; Marine & Freshwater Biology ; Veterinary Sciences
研究领域[WOS]: Fisheries ; Immunology ; Marine & Freshwater Biology ; Veterinary Sciences
摘要: Interferon (IFN) can induce an antiviral state via interferon-regulatory transcription factors (IRFs), which bind to and control genes directed by the interferon-stimulated response element (ISRE). Here we describe a fish IRF, termed CaIRF7, cloned from a subtractive cDNA library which is constructed with mRNAs obtained from crucian carp (Carassius auratus L.) blastulae embryonic (CAB) cells infected by UV-inactivated GCHV and mock-infected cells. CaIRF7 cDNA was found to be 1816 bp in length, with a 42 bp 5' UTR and a 508 bp 3' UTR. The open reading frame translates into 421 amino acids in which a DNA-binding domain (DBD) containing the repeated tryptophan motif and IRFs association domain have been identified. Like chicken GgIRF3, CaIRF7 was most similar to mammalian IRF7 with 27 to 30% identity overall and some 37% identity in their DBDs. A single transcript of 1.9 kb was detected in virally induced CAB cells by virtual Northern blotting. RT-PCR analysis revealed a wide tissue distribution of CaIRF7 constitutive expression, with detectable transcript in non-infected CAB cells and various tissues of healthy crucian carp. In addition, CaIRF7 expression was differentially increased by stimulation of the CAB cells with active GCHV, UV-inactivated GCHV or CAB IFN, indicating that the activation of CaIRF7 was directly regulated by IFN. (C) 2003 Published by Elsevier Ltd.
英文摘要: Interferon (IFN) can induce an antiviral state via interferon-regulatory transcription factors (IRFs), which bind to and control genes directed by the interferon-stimulated response element (ISRE). Here we describe a fish IRF, termed CaIRF7, cloned from a subtractive cDNA library which is constructed with mRNAs obtained from crucian carp (Carassius auratus L.) blastulae embryonic (CAB) cells infected by UV-inactivated GCHV and mock-infected cells. CaIRF7 cDNA was found to be 1816 bp in length, with a 42 bp 5' UTR and a 508 bp 3' UTR. The open reading frame translates into 421 amino acids in which a DNA-binding domain (DBD) containing the repeated tryptophan motif and IRFs association domain have been identified. Like chicken GgIRF3, CaIRF7 was most similar to mammalian IRF7 with 27 to 30% identity overall and some 37% identity in their DBDs. A single transcript of 1.9 kb was detected in virally induced CAB cells by virtual Northern blotting. RT-PCR analysis revealed a wide tissue distribution of CaIRF7 constitutive expression, with detectable transcript in non-infected CAB cells and various tissues of healthy crucian carp. In addition, CaIRF7 expression was differentially increased by stimulation of the CAB cells with active GCHV, UV-inactivated GCHV or CAB IFN, indicating that the activation of CaIRF7 was directly regulated by IFN. (C) 2003 Published by Elsevier Ltd.
关键词[WOS]: SEQUENCE-BINDING-PROTEIN ; TRANSCRIPTION ACTIVATION ; GENE INDUCTION ; ALPHA GENES ; EXPRESSION ; VIRUS ; DISTINCT ; FAMILY ; IRF-3 ; INFECTION
语种: 英语
WOS记录号: WOS:000186734400008
ISSN号: 1050-4648
Citation statistics:
内容类型: 期刊论文
URI标识: http://ir.ihb.ac.cn/handle/152342/9610
Appears in Collections:中科院水生所知识产出(2009年前)_期刊论文

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作者单位: 1.Chinese Acad Sci, Inst Hydrobiol, State Key Lab Freshwater Ecol & Biotechnol, Wuhan Ctr Dev Biol, Wuhan 430072, Hubei Province, Peoples R China

Recommended Citation:
Yi-bing Zhang; Cheng-yu Hu; Jing Zhang; Guo-ping Huang; Li-hua Wei; Qi-ya Zhang; Jian-fang Gui.Molecular cloning and characterization of crucian carp (Carassius auratus L.) interferon regulatory factor 7,FISH & SHELLFISH IMMUNOLOGY,2003,15(5):453-466
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