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Molecular characterization and IFN signal pathway analysis of Carassius auratus CaSTAT1 identified from the cultured cells in response to virus infection
Yibing Zhang; Jianfang Gui; Gui, HF, Chinese Acad Sci, State Key Lab Freshwater Ecol & Biotechnol, Dept Fish Genet & Breeding, Inst Hydrobiol,Wuhan Ctr Dev Biol, Wuhan 430072, Peoples R China
2004
Source PublicationDEVELOPMENTAL AND COMPARATIVE IMMUNOLOGY
Volume28Issue:3Pages:211-227
AbstractType I interferon (IFN) exerts its pleiotropic effects mainly through the JAK-STAT signaling pathway, which is presently best described in mammals. By subtractive suppression hybridization, two fish signaling factors, JAK1 and STAT1, had been identified in the IFN-induced crucian carp Carassius auratus L. blastulae embryonic (CAB) cells after treatment with UV-inactivated grass carp hemorrhagic virus (GCHV). Further, the full-length cDNA of STAT1, termed CaSTAT1, was obtained. It contains 2926 bp and encodes a protein of 718 aa. CaSTAT1 is most similar to rat STAT1 with 59% identity overall and displays all highly conserved domains that the STAT family possesses. Like human STAT1beta, it lacks the C-terminus acting as transcriptional activation domain in mammals. By contrast, only a single transcript was detected in virus-induced CAB cells. Expression analysis showed that CaSTAT1 could be activated by stimulation of CAB cells with poly I:C, active GCHV, UV-inactivated GCHV or CAB IFN, and displayed diverse expression patterns similar to that of mammalian STATI. Additionally, the expression of an antiviral gene CaMx1 was also induced under the same conditions, and expression difference between CaSTAT1 and CaMx1 was revealed by induction of CAB IFN. These results provide molecular evidence supporting the notion that the fish IFN signaling transduction pathway is similar to that in mammals. Fish IFN exerts its multiple functions, at least antiviral action, through a JAK-STAT pathway. (C) 2004 Elsevier Ltd. All rights reserved.
KeywordJanus Kinase-Signal Transducer And Activator Of Transcription Signal Pathway Stat1 Cdna Cloning Expression Analysis Crucian Carp Blastulae Embryonic Cells Grass Carp Hemorrhagic Virus
DepartmentChinese Acad Sci, State Key Lab Freshwater Ecol & Biotechnol, Dept Fish Genet & Breeding, Inst Hydrobiol,Wuhan Ctr Dev Biol, Wuhan 430072, Peoples R China
Subject AreaImmunology ; Zoology
Indexed BySCI
WOS IDWOS:000188375700004
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Cited Times:52[WOS]   [WOS Record]     [Related Records in WOS]
Document Type期刊论文
Identifierhttp://ir.ihb.ac.cn/handle/152342/9576
Collection期刊论文
Corresponding AuthorGui, HF, Chinese Acad Sci, State Key Lab Freshwater Ecol & Biotechnol, Dept Fish Genet & Breeding, Inst Hydrobiol,Wuhan Ctr Dev Biol, Wuhan 430072, Peoples R China
Recommended Citation
GB/T 7714
Yibing Zhang,Jianfang Gui,Gui, HF, Chinese Acad Sci, State Key Lab Freshwater Ecol & Biotechnol, Dept Fish Genet & Breeding, Inst Hydrobiol,Wuhan Ctr Dev Biol, Wuhan 430072, Peoples R China. Molecular characterization and IFN signal pathway analysis of Carassius auratus CaSTAT1 identified from the cultured cells in response to virus infection[J]. DEVELOPMENTAL AND COMPARATIVE IMMUNOLOGY,2004,28(3):211-227.
APA Yibing Zhang,Jianfang Gui,&Gui, HF, Chinese Acad Sci, State Key Lab Freshwater Ecol & Biotechnol, Dept Fish Genet & Breeding, Inst Hydrobiol,Wuhan Ctr Dev Biol, Wuhan 430072, Peoples R China.(2004).Molecular characterization and IFN signal pathway analysis of Carassius auratus CaSTAT1 identified from the cultured cells in response to virus infection.DEVELOPMENTAL AND COMPARATIVE IMMUNOLOGY,28(3),211-227.
MLA Yibing Zhang,et al."Molecular characterization and IFN signal pathway analysis of Carassius auratus CaSTAT1 identified from the cultured cells in response to virus infection".DEVELOPMENTAL AND COMPARATIVE IMMUNOLOGY 28.3(2004):211-227.
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