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学科主题: Immunology; Zoology
题名: Molecular characterization and IFN signal pathway analysis of Carassius auratus CaSTAT1 identified from the cultured cells in response to virus infection
作者: Zhang, YB; Gui, HF
通讯作者: Gui, HF, Chinese Acad Sci, State Key Lab Freshwater Ecol & Biotechnol, Dept Fish Genet & Breeding, Inst Hydrobiol,Wuhan Ctr Dev Biol, Wuhan 430072, Peoples R China
关键词: Janus kinase-signal transducer and activator of transcription signal pathway ; STAT1 ; cDNA cloning ; expression analysis ; Crucian carp blastulae embryonic cells ; grass carp hemorrhagic virus
刊名: DEVELOPMENTAL AND COMPARATIVE IMMUNOLOGY
发表日期: 2004-03-01
DOI: 10.1016/S0145-305X(03)00138-1
卷: 28, 期:3, 页:211-227
收录类别: SCI
文章类型: Article
部门归属: Chinese Acad Sci, State Key Lab Freshwater Ecol & Biotechnol, Dept Fish Genet & Breeding, Inst Hydrobiol,Wuhan Ctr Dev Biol, Wuhan 430072, Peoples R China
WOS标题词: Science & Technology ; Life Sciences & Biomedicine
类目[WOS]: Immunology ; Zoology
研究领域[WOS]: Immunology ; Zoology
摘要: Type I interferon (IFN) exerts its pleiotropic effects mainly through the JAK-STAT signaling pathway, which is presently best described in mammals. By subtractive suppression hybridization, two fish signaling factors, JAK1 and STAT1, had been identified in the IFN-induced crucian carp Carassius auratus L. blastulae embryonic (CAB) cells after treatment with UV-inactivated grass carp hemorrhagic virus (GCHV). Further, the full-length cDNA of STAT1, termed CaSTAT1, was obtained. It contains 2926 bp and encodes a protein of 718 aa. CaSTAT1 is most similar to rat STAT1 with 59% identity overall and displays all highly conserved domains that the STAT family possesses. Like human STAT1beta, it lacks the C-terminus acting as transcriptional activation domain in mammals. By contrast, only a single transcript was detected in virus-induced CAB cells. Expression analysis showed that CaSTAT1 could be activated by stimulation of CAB cells with poly I:C, active GCHV, UV-inactivated GCHV or CAB IFN, and displayed diverse expression patterns similar to that of mammalian STATI. Additionally, the expression of an antiviral gene CaMx1 was also induced under the same conditions, and expression difference between CaSTAT1 and CaMx1 was revealed by induction of CAB IFN. These results provide molecular evidence supporting the notion that the fish IFN signaling transduction pathway is similar to that in mammals. Fish IFN exerts its multiple functions, at least antiviral action, through a JAK-STAT pathway. (C) 2004 Elsevier Ltd. All rights reserved.
英文摘要: Type I interferon (IFN) exerts its pleiotropic effects mainly through the JAK-STAT signaling pathway, which is presently best described in mammals. By subtractive suppression hybridization, two fish signaling factors, JAK1 and STAT1, had been identified in the IFN-induced crucian carp Carassius auratus L. blastulae embryonic (CAB) cells after treatment with UV-inactivated grass carp hemorrhagic virus (GCHV). Further, the full-length cDNA of STAT1, termed CaSTAT1, was obtained. It contains 2926 bp and encodes a protein of 718 aa. CaSTAT1 is most similar to rat STAT1 with 59% identity overall and displays all highly conserved domains that the STAT family possesses. Like human STAT1beta, it lacks the C-terminus acting as transcriptional activation domain in mammals. By contrast, only a single transcript was detected in virus-induced CAB cells. Expression analysis showed that CaSTAT1 could be activated by stimulation of CAB cells with poly I:C, active GCHV, UV-inactivated GCHV or CAB IFN, and displayed diverse expression patterns similar to that of mammalian STATI. Additionally, the expression of an antiviral gene CaMx1 was also induced under the same conditions, and expression difference between CaSTAT1 and CaMx1 was revealed by induction of CAB IFN. These results provide molecular evidence supporting the notion that the fish IFN signaling transduction pathway is similar to that in mammals. Fish IFN exerts its multiple functions, at least antiviral action, through a JAK-STAT pathway. (C) 2004 Elsevier Ltd. All rights reserved.
关键词[WOS]: PUFFERFISH TETRAODON-FLUVIATILIS ; INTERFERON REGULATORY FACTOR ; TRANSCRIPTION FACTOR ISGF-3 ; NF-KAPPA-B ; RAINBOW-TROUT ; GENE-EXPRESSION ; SERINE PHOSPHORYLATION ; GENOMIC ORGANIZATION ; TARGETED DISRUPTION ; STAT PROTEIN
语种: 英语
WOS记录号: WOS:000188375700004
ISSN号: 0145-305X
Citation statistics:
内容类型: 期刊论文
URI标识: http://ir.ihb.ac.cn/handle/152342/9576
Appears in Collections:中科院水生所知识产出(2009年前)_期刊论文

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作者单位: 1.Chinese Acad Sci, State Key Lab Freshwater Ecol & Biotechnol, Dept Fish Genet & Breeding, Inst Hydrobiol,Wuhan Ctr Dev Biol, Wuhan 430072, Peoples R China

Recommended Citation:
Yibing Zhang; Jianfang Gui.Molecular characterization and IFN signal pathway analysis of Carassius auratus CaSTAT1 identified from the cultured cells in response to virus infection,DEVELOPMENTAL AND COMPARATIVE IMMUNOLOGY,2004,28(3):211-227
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