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Identification and expression analysis of two IFN-inducible genes in crucian carp (Carassius auratus L.)
Zhang, YB; Gui, HF; Gui, HF, Chinese Acad Sci, Inst Hydrobiol, Wuhan Ctr Dev Biol,Dept Fish Genet & Breeding, State Key Lab Freshwater Ecol & Biotechnol, Wuhan 430072, Hubei Province, Peoples R China
2004
Source PublicationGENE
ISSN0378-1119
Volume325Issue:*Pages:43-51
AbstractInterferon (IFN) exerts its antiviral effects mainly through activation of a subset of IFN-stimulated genes (ISG), but relatively few of fish ISGs have been isolated and characterized so far. Here, we report two fish ISGs, termed CaIF158 and CaIF156, cloned from a subtractive cDNA library constructed with mRNAs obtained from crucian carp (Carassius auratus L.) blastulae embryonic (CAB) cells infected by UV-inactivated GCHV and mock-infected cells. Database search revealed that both ISGs had a high-level homology with all members of a well conserved gene family with multiple tetratricopeptide repeat (TPR) motifs, including human IF160, IF158, IF156, IFI54 and their homologues in some other mammalian species. The transcripts of CaIF158 and CaIF156 were undetectable in CAB cells but could be induced by active GCHV, UV-inactivated GCHV or CAB IFN. Analysis of expression difference between them and IFN signal factors, CaSTAT1 and CaIRF7, indicated that their transcriptions were mediated possibly through JAK-STAT signal pathway, which was further supported by the induction analysis in UV-inactivated GCHV infected, IFN-treated and untreated cells in the presence or absence of cycloheximide (CHX), a potent inhibitor of protein synthesis. In addition, a pufferfish (Fugu rubrides) DNA sequence representing putative FrIFI56 was also revealed when CalF158 and CalF156 were used to search the pufferfish genome database. Phylogenetic analysis showed that these fish ISGs form a unique clad independent of mammalian homologues, reflecting a distant evolutionary relationship from mammals. These studies identified the first teleost IFI56 and IFI58 orthologues. (C) 2003 Elsevier B.V All rights reserved.
KeywordIfn-stimulated Genes Qsg) Tetratricopeptide Repeat (Tpr) Ifi56 Ifi58 Grass Carp Hemorrhage Virus (Gchv) Antiviral-relevant Genes
DepartmentChinese Acad Sci, Inst Hydrobiol, Wuhan Ctr Dev Biol,Dept Fish Genet & Breeding, State Key Lab Freshwater Ecol & Biotechnol, Wuhan 430072, Hubei Province, Peoples R China
Subject AreaGenetics & Heredity
Indexed BySCI
WOS IDWOS:000188291200005
Citation statistics
Cited Times:28[WOS]   [WOS Record]     [Related Records in WOS]
Document Type期刊论文
Identifierhttp://ir.ihb.ac.cn/handle/152342/9574
Collection期刊论文
Corresponding AuthorGui, HF, Chinese Acad Sci, Inst Hydrobiol, Wuhan Ctr Dev Biol,Dept Fish Genet & Breeding, State Key Lab Freshwater Ecol & Biotechnol, Wuhan 430072, Hubei Province, Peoples R China
Recommended Citation
GB/T 7714
Zhang, YB,Gui, HF,Gui, HF, Chinese Acad Sci, Inst Hydrobiol, Wuhan Ctr Dev Biol,Dept Fish Genet & Breeding, State Key Lab Freshwater Ecol & Biotechnol, Wuhan 430072, Hubei Province, Peoples R China. Identification and expression analysis of two IFN-inducible genes in crucian carp (Carassius auratus L.)[J]. GENE,2004,325(*):43-51.
APA Zhang, YB,Gui, HF,&Gui, HF, Chinese Acad Sci, Inst Hydrobiol, Wuhan Ctr Dev Biol,Dept Fish Genet & Breeding, State Key Lab Freshwater Ecol & Biotechnol, Wuhan 430072, Hubei Province, Peoples R China.(2004).Identification and expression analysis of two IFN-inducible genes in crucian carp (Carassius auratus L.).GENE,325(*),43-51.
MLA Zhang, YB,et al."Identification and expression analysis of two IFN-inducible genes in crucian carp (Carassius auratus L.)".GENE 325.*(2004):43-51.
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