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学科主题: Microbiology
题名: alr0117, a two-component histidine kinase gene, is involved in heterocyst development in Anabaena sp PCC 7120
作者: Ning, DG; Xu, XD
通讯作者: Xu, XD, Chinese Acad Sci, Inst Hydrobiol, Wuhan 430072, Peoples R China
关键词: CalA ; ftsZ ; DNA-binding protein ; Anabaena
刊名: MICROBIOLOGY-SGM
发表日期: 2004-02-01
DOI: 10.1099/mic.0.26747-0
卷: 150, 期:0, 页:447-453
收录类别: SCI
文章类型: Article
部门归属: Chinese Acad Sci, Inst Hydrobiol, Wuhan 430072, Peoples R China
WOS标题词: Science & Technology ; Life Sciences & Biomedicine
类目[WOS]: Microbiology
研究领域[WOS]: Microbiology
摘要: Anabaena sp. PCC; 7120 was mutagenized by transposon Tn5-1087b, generating a mutant whose heterocysts lack the envelope polysaccharide layer. The transposon was located between nucleotides 342 and 343 of alr0117, a 918 bp gene encoding a histidine kinase for a two-component regulatory system. Complementation of the mutant with a DNA fragment containing alr0117 and targeted inactivation of the gene confirmed that alr0117 is involved in heterocyst development. RT-PCR showed that alr0117 was constitutively expressed in the presence or absence of a combined-nitrogen source. hepA and patB, the two genes turned on during wild-type heterocyst development, were no longer activated in an alr0117-null mutant. The two-component signal transduction system involving alr0117 may control the formation of the envelope polysaccharide layer and certain late events essential to the function of heterocysts.
英文摘要: Anabaena sp. PCC; 7120 was mutagenized by transposon Tn5-1087b, generating a mutant whose heterocysts lack the envelope polysaccharide layer. The transposon was located between nucleotides 342 and 343 of alr0117, a 918 bp gene encoding a histidine kinase for a two-component regulatory system. Complementation of the mutant with a DNA fragment containing alr0117 and targeted inactivation of the gene confirmed that alr0117 is involved in heterocyst development. RT-PCR showed that alr0117 was constitutively expressed in the presence or absence of a combined-nitrogen source. hepA and patB, the two genes turned on during wild-type heterocyst development, were no longer activated in an alr0117-null mutant. The two-component signal transduction system involving alr0117 may control the formation of the envelope polysaccharide layer and certain late events essential to the function of heterocysts.
关键词[WOS]: SP STRAIN PCC-7120 ; PCC 7120 ; DIFFERENTIATION ; EXPRESSION ; SEQUENCE ; PATTERN ; GLYCOLIPIDS ; ENVELOPE
语种: 英语
WOS记录号: WOS:000188841500023
ISSN号: 1350-0872
Citation statistics:
内容类型: 期刊论文
URI标识: http://ir.ihb.ac.cn/handle/152342/9568
Appears in Collections:中科院水生所知识产出(2009年前)_期刊论文

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作者单位: 1.Chinese Acad Sci, Inst Hydrobiol, Wuhan 430072, Peoples R China

Recommended Citation:
Ning, DG; Xu, XD.alr0117, a two-component histidine kinase gene, is involved in heterocyst development in Anabaena sp PCC 7120,MICROBIOLOGY-SGM,2004,150():447-453
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