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alr0117, a two-component histidine kinase gene, is involved in heterocyst development in Anabaena sp PCC 7120
Ning, DG; Xu, XD; Xu, XD, Chinese Acad Sci, Inst Hydrobiol, Wuhan 430072, Peoples R China
2004-02-01
Source PublicationMICROBIOLOGY-SGM
ISSN1350-0872
Volume150Issue:0Pages:447-453
AbstractAnabaena sp. PCC; 7120 was mutagenized by transposon Tn5-1087b, generating a mutant whose heterocysts lack the envelope polysaccharide layer. The transposon was located between nucleotides 342 and 343 of alr0117, a 918 bp gene encoding a histidine kinase for a two-component regulatory system. Complementation of the mutant with a DNA fragment containing alr0117 and targeted inactivation of the gene confirmed that alr0117 is involved in heterocyst development. RT-PCR showed that alr0117 was constitutively expressed in the presence or absence of a combined-nitrogen source. hepA and patB, the two genes turned on during wild-type heterocyst development, were no longer activated in an alr0117-null mutant. The two-component signal transduction system involving alr0117 may control the formation of the envelope polysaccharide layer and certain late events essential to the function of heterocysts.; Anabaena sp. PCC; 7120 was mutagenized by transposon Tn5-1087b, generating a mutant whose heterocysts lack the envelope polysaccharide layer. The transposon was located between nucleotides 342 and 343 of alr0117, a 918 bp gene encoding a histidine kinase for a two-component regulatory system. Complementation of the mutant with a DNA fragment containing alr0117 and targeted inactivation of the gene confirmed that alr0117 is involved in heterocyst development. RT-PCR showed that alr0117 was constitutively expressed in the presence or absence of a combined-nitrogen source. hepA and patB, the two genes turned on during wild-type heterocyst development, were no longer activated in an alr0117-null mutant. The two-component signal transduction system involving alr0117 may control the formation of the envelope polysaccharide layer and certain late events essential to the function of heterocysts.
SubtypeArticle
KeywordCala Ftsz Dna-binding Protein Anabaena
DepartmentChinese Acad Sci, Inst Hydrobiol, Wuhan 430072, Peoples R China
Subject AreaMicrobiology
DOI10.1099/mic.0.26747-0
WOS HeadingsScience & Technology ; Life Sciences & Biomedicine
Indexed BySCI
Language英语
WOS Research AreaMicrobiology
WOS SubjectMicrobiology
WOS IDWOS:000188841500023
WOS KeywordSP STRAIN PCC-7120 ; PCC 7120 ; DIFFERENTIATION ; EXPRESSION ; SEQUENCE ; PATTERN ; GLYCOLIPIDS ; ENVELOPE
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Document Type期刊论文
Identifierhttp://ir.ihb.ac.cn/handle/152342/9568
Collection期刊论文
Corresponding AuthorXu, XD, Chinese Acad Sci, Inst Hydrobiol, Wuhan 430072, Peoples R China
AffiliationChinese Acad Sci, Inst Hydrobiol, Wuhan 430072, Peoples R China
Recommended Citation
GB/T 7714
Ning, DG,Xu, XD,Xu, XD, Chinese Acad Sci, Inst Hydrobiol, Wuhan 430072, Peoples R China. alr0117, a two-component histidine kinase gene, is involved in heterocyst development in Anabaena sp PCC 7120[J]. MICROBIOLOGY-SGM,2004,150(0):447-453.
APA Ning, DG,Xu, XD,&Xu, XD, Chinese Acad Sci, Inst Hydrobiol, Wuhan 430072, Peoples R China.(2004).alr0117, a two-component histidine kinase gene, is involved in heterocyst development in Anabaena sp PCC 7120.MICROBIOLOGY-SGM,150(0),447-453.
MLA Ning, DG,et al."alr0117, a two-component histidine kinase gene, is involved in heterocyst development in Anabaena sp PCC 7120".MICROBIOLOGY-SGM 150.0(2004):447-453.
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