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Broad host range plasmid-based gene transfer system in the cyanobacterium Gloeobacter violaceus which lacks thylakoids
Guo, HT; Xu, XD; Xu, XD, Chinese Acad Sci, Inst Hydrobiol, Wuhan 430072, Peoples R China
2004
Source PublicationPROGRESS IN NATURAL SCIENCE
ISSN1002-0071
Volume14Issue:1Pages:31-35
AbstractGloeobacter violaceus, a cyanobacterium lack of thylakoids, is refractory to genetic manipulations because its cells are enveloped by a thick gelatinous sheath and in colonial form. In this study, a large number of single cells were obtained by repeated pumping with a syringe with the gelatinous sheath removed. And an exogenous broad host range plasmid pKT210 was conjugatively transferred into G. violaceus. Analyses with dot-blot hybridization and restriction mapping showed that the exogenous plasmid pKT210 had been introduced into G. violaceus and stably maintained with no alteration in its structure. pKT210 extracted from G. violaceus exconjugants could be transformed into the mcr - mrr - E. coli strain DH10B but not the mcr(+) mrr(+) strain DH5alpha, which suggests that a methylase system may be present in G. violaceus.; Gloeobacter violaceus, a cyanobacterium lack of thylakoids, is refractory to genetic manipulations because its cells are enveloped by a thick gelatinous sheath and in colonial form. In this study, a large number of single cells were obtained by repeated pumping with a syringe with the gelatinous sheath removed. And an exogenous broad host range plasmid pKT210 was conjugatively transferred into G. violaceus. Analyses with dot-blot hybridization and restriction mapping showed that the exogenous plasmid pKT210 had been introduced into G. violaceus and stably maintained with no alteration in its structure. pKT210 extracted from G. violaceus exconjugants could be transformed into the mcr - mrr - E. coli strain DH10B but not the mcr(+) mrr(+) strain DH5alpha, which suggests that a methylase system may be present in G. violaceus.
SubtypeArticle
KeywordGloeobacter Violaceus Conjugation Pkt210
DepartmentChinese Acad Sci, Inst Hydrobiol, Wuhan 430072, Peoples R China
Subject AreaMultidisciplinary Sciences
WOS HeadingsScience & Technology ; Technology
Indexed BySCI
Language英语
WOS Research AreaMaterials Science ; Science & Technology - Other Topics
WOS SubjectMaterials Science, Multidisciplinary ; Multidisciplinary Sciences
WOS IDWOS:000188789300006
WOS KeywordESCHERICHIA-COLI ; CONJUGATIVE TRANSFER ; TRANSFORMATION ; VECTORS ; DNA
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Cited Times:3[WOS]   [WOS Record]     [Related Records in WOS]
Document Type期刊论文
Identifierhttp://ir.ihb.ac.cn/handle/152342/9560
Collection期刊论文
Corresponding AuthorXu, XD, Chinese Acad Sci, Inst Hydrobiol, Wuhan 430072, Peoples R China
AffiliationChinese Acad Sci, Inst Hydrobiol, Wuhan 430072, Peoples R China
Recommended Citation
GB/T 7714
Guo, HT,Xu, XD,Xu, XD, Chinese Acad Sci, Inst Hydrobiol, Wuhan 430072, Peoples R China. Broad host range plasmid-based gene transfer system in the cyanobacterium Gloeobacter violaceus which lacks thylakoids[J]. PROGRESS IN NATURAL SCIENCE,2004,14(1):31-35.
APA Guo, HT,Xu, XD,&Xu, XD, Chinese Acad Sci, Inst Hydrobiol, Wuhan 430072, Peoples R China.(2004).Broad host range plasmid-based gene transfer system in the cyanobacterium Gloeobacter violaceus which lacks thylakoids.PROGRESS IN NATURAL SCIENCE,14(1),31-35.
MLA Guo, HT,et al."Broad host range plasmid-based gene transfer system in the cyanobacterium Gloeobacter violaceus which lacks thylakoids".PROGRESS IN NATURAL SCIENCE 14.1(2004):31-35.
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